| 摘要: |
| 研究了卵龄、电脉冲参数、氯化锶、亚胺环己酮（CHX）对小鼠卵母细胞电激活及孤雌发育效果的影响。结果表明，卵龄为17 h的小鼠卵母细胞在场强1.0 kV/cm，脉冲宽度30 μs，2次电脉冲，激活液中含Ca2+、Mg2+和Sr2+的条件下激活后，再于含CHX的培养液中培养，可较好地激活卵母细胞，提高体外孤雌胚的发育率,激活率及桑囊胚率分别可达83.3%和57.5%。 |
| 关键词: 小鼠 卵母细胞 电激活 氯化锶 亚胺环己酮 |
| DOI： |
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| 基金项目:国家“863”高技术研究发展计划项目（2001AA213081） |
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| Effects of different factor on electroactivation and parthenogenesis of mouse oocytes |
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| Abstract: |
| This study was designed to evaluate the effect of age,electric pulse parameter,SrCl2,cycloheximide (CHX) on electroactivation and parthenogenesis of mouse oocytes.The results showed that mouse oocytes which were cultured in CHX-contained CZB medium in vitro after electric activation could obtain higher activated percentage and developmental percentage.Oocytes came from mouse 17 hours after being injected with HCG.Medium of activation contained Ca2+、Mg2+ and Sr2+.The condition of electroactivation was field strength 1.0 kV/cm,pulse duration 30 μs,two times pulses.The percentage of activation and morulae/blastocysts could reach 83.3% and 57.5% respectively. |
| Key words: mouse oocytes electroactivation SrCl2 cycloheximide |
