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中国小麦条锈菌4个流行小种的RAPD标记
曹丽华1, 康振生1, 赵 杰1
西北农林科技大学 植保学院 生物技术中心
摘要:
以210条随机引物,对目前中国小麦条锈菌(Puccinia striiformisf.sp.Tritici)的主要优势菌系进行了RAPD片段的规模筛选,寻找到了条中31号、条中29号、条中23号、水源类型4个流行生理小种的特异性RAPD标记。此结果表明,通过寻找小麦条锈菌生理小种的特异性RAPD片段,能够建立起中国小麦条锈菌生理小种的分子检测体系。
关键词:  小麦条锈菌  RAPD  分子标记
DOI:
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基金项目:国家“973”计划项目(G200016201)
RAPD markers of Puccinia striiformis f.sp.tritici in China
Abstract:
Rapid identification of the races of Puccinia striiformis f.sp.tritici by molecular technique was very important to monitor and control wheat stripe rust in China.In this paper,five physiological races of Puccinia striiformis f.sp.tritici were analysed with 210 primers by RAPD technique.The special RAPD fragments of CY31,CY29,CY23 and Shuiyuan pathotype were founded successfully.The results here indicates that a molecular identification system for the races of Puccinia striiformis f.sp.tritici in China could be established through cosmically searching special RAPD fragments of different races.
Key words:  wheat stripe rust  RAPD  molecular marker

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