| 摘要: |
| 应用RT-PCR法，对分离于当地典型发病乌鸡群的IBDV(WJ株)进行了VP2基因的克隆与序列分析，并和相关毒株进行了比较。结果表明，WJ株与标准血清Ⅰ型STC株的核苷酸和氨基酸的同源性只有91.39％和92.74％，而与超强毒株UK661和从当地鸡群中分离的超强毒株HN01株的核苷酸和氨基酸的同源性则较高，其中，与其核苷酸同源性分别为95.43％和95.87％，与其氨基酸同源性均为96.77％。且该毒株的VP2基因序列完全具备超强毒株的主要特征。由此说明，分离于当地发病乌鸡群的IBDV为超强毒株，并和当地流行的鸡IBD超强毒株有较高的同源性，但也有明显的差异。 |
| 关键词: 乌鸡 IBDV VP2基因 克隆 序列分析 |
| DOI： |
| 分类号: |
| 基金项目:河南省骨干教师资助项目(2003056) |
|
| Cloning and sequence analysis of the VP2 gene of IBDV isolated from dark-bone chicken |
|
|
| Abstract: |
| The VP2 gene of IBDV (WJ strain) isolated from dark-bone chicken were amplified by RT-PCR and cloned into pMD18-T vector to analysed sequence.Sequence comparison with standard I sterotype IBDV (STC),the homology of the nucleotide and amino acid were only 91.39% and 92.74%,separately;But comparison with very-virulent IBDV (vv IBDV) UK661 and HN01,the homology of nucleotide were 95.43% and 95.87%,96.77%;the homology of amino acid all were 96.77%.Moreover,the VP2 gene sequence of WJ strain possessed completely the characteristics of vv IBDV.The results showed that WJ strain was a very-virulent IBDV. |
| Key words: dark-bone chicken IBDV VP2 gene cloning sequence analysis |
