| 摘要: |
| 利用普通及重组PCR技术克隆了奶牛β-酪蛋白基因(CSN2)5′调控成分(2 826 bp)和3′调控成分(620 bp)。前者包括5′上游调控序列、第一外显子及部分第一内含子；后者主要包括最后一个不翻译的外显子和3′侧翼序列。纯化PCR产物通过pMD 18-T Vector亚克隆后测序并进行软件分析的结果表明，2个克隆片断与奶牛CSN2相应区域同源性分别为99.0％和98.0％，并且包含有全部的CSN2表达核心调控序列和多个转录、翻译因子的结合位点。 |
| 关键词: 奶牛 CSN2 调控区 PCR 序列分析 |
| DOI： |
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| 基金项目:国家“863”高技术项目(2001AA213081) |
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| Molecular cloning and sequence analysis of bovine beta-casein gene 5′,3′control region |
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| Abstract: |
| 5′and 3′control region of bovine CSN2 were partially cloned by normal and recombinant PCR.The former consisted in all of the 5′upstream sequence,the first exon and part of first intron,while the later included the last exon and 3′flanking fragment,The purified PCR products were sub-cloned to pMD 18-T Vector,then sequenced correspondently and analysed by bio-soft.The results indicated that the twe cloned fragments have the homology of 99.0% and 98.0% respectively with the comparable region of bovine CSN2.Moreover,all the vital regulatory elements and many factors binding sites were found in the two fragments.The above suggested that the expressing vector,constructed by the two cloned fragments and work under the control model of endogenous CSN2,could direct the target gene to express specifically and efficiently in mammary gland. |
| Key words: cow CSN2 control region PCR sequence analysis |
