| 摘要: |
| 以胡萝卜无菌幼苗的下胚轴为外植体，通过携带CTB基因的根癌农杆菌的介导进行转化，在Kan 50 mg/L和Carb 500 mg/L的MS3-1固体培养基上诱导形成胚性愈伤组织，转至Kan 100 mg/L和Carb 100 mg/L的MS0固体培养基上形成植株，同时还进行了以经过预处理的下胚轴为外植体进行转化的试验，发现低温处理4 d的下胚轴的抗性愈伤诱导率较高。经PCR检测、PCR-Southern杂交检测以后，表明CTB基因已整合进胡萝卜的基因组中。RT PCR检测初步证明了CTB基因的转录。 |
| 关键词: 霍乱毒素B亚基（CTB基因） 胡萝卜 遗传转化 |
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| Genetic transformation of CT-B on carrot |
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| Abstract: |
| The hypocotyl of carrot were infected with Agrobacterium tumefaciens containing CTB gene,the explants were transferred to MS3-1 selection media which contained Kan 50 mg/L and carb 500 mg/L to induce embryogenic callus.Then the callus were transferred to MS0 media to form plantlet,and it was found that the transgenic rate of hypocotyls which were treated of low temperature were higher in all treatments.PCR,PCR-Southern detection of carrot DNA confirmed that the CTB gene had been introduced into the plant genome.RT-PCR detection showed primarily the transcription of CTB. |
| Key words: cholera B subunit (CTB gene) carrot genetic transformation |
