| 摘要: |
| 分离培养黑熊皮肤成纤维细胞，传3代，分别经血清饥饿2～6 d和10 g/L Nocodazole处理24 h后，用流式细胞仪分析2个处理组和对照组细胞的周期相，以研究黑熊成纤维细胞同步化处理后的细胞周期时相的变化，探讨核移植供体细胞的细胞周期对核移植胚胎发育的影响。结果显示，血清饥饿组、Nocodazole处理组及对照组的G0+G1期成纤维细胞的百分率分别为（85.05±7.00）%,(45.9±3.7)%和(59.3±6.7)%；G2/M期细胞的百分率分别为（10.4±6.8）%,（46.3±4.2）%及（23.05±1.05）%。表明血清饥饿法能较好地使黑熊成纤维细胞同步于G0和G1期；Nocodazole处理可以使细胞较大程度同步于G2和M期。 |
| 关键词: 成纤维细胞 细胞周期同步化 流式细胞仪 黑熊 |
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| Effect of synchronization-treated bear fibroblast cells cycle analysed by flow cytometer |
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| Abstract: |
| After 3 passages of the primary black bear fibroblast cells,the cells were serum starvated for 2-6 days or treated with 10 g/L Nocodazole for 24 h,then calculation of percentages of the treated cells and the control cells in G0,G1 and G2+M phases of the cell cycle by using flow cytometer was conducted respectively,to investigate the cell cycle characteristics of different treatment cultured fibroblast cells.The results showed that:G0+G1 cell percentage of the serum starvation,Nocodazole treatment,and control are (85.05±7.00)%,(45.9±3.7)% and (59.3±6.7)%,respectively.G2/M are (10.4±6.8)%,(46.3±4.2)% and (23.05±1.05)%，respectively.Black bear fibroblast cell treated with serum starvation for 5 days contained higher (P<0.05) percentages of G0+G1,whereas cells treated with nocodazole contained higher percentage of G2+M. |
| Key words: fibroblast cell synchronization of the cell cycle flow cytometer black bear |
