| 摘要: |
| 研究了细胞色素P450含量及NADPH-细胞色素P450还原酶活性与苦皮藤素V对粘虫(Mythimna separata)和小地老虎(Agrotis ypsilon)选择毒性的关系。结果表明，对照组小地老虎中肠细胞色素P450含量及NADPH-细胞色素P450还原酶活性均显著高于粘虫。苦皮藤素V处理后，小地老虎和粘虫中肠细胞色素P450含量均显著高于对照，粘虫中毒抽搐期及失水期中肠细胞色素P450含量分别比对照升高1.46和2.26倍，NADPH-细胞色素P450还原酶活性分别比对照升高1.26和2.56倍；处理组小地老虎中肠细胞色素P450含量比对照升高0.39倍，NADPH-细胞色素P450还原酶活性略高于对照。由此认为，中肠细胞色素P450含量及NADPH-细胞色素P450还原酶活性与苦皮藤素V对粘虫和小地老虎产生选择毒杀活性有着重要的关系。并且苦皮藤素v对细胞色素P450酶系具有一定的诱导作用，但对不同昆虫以及同一种昆虫中P450酶系的不同组分诱导作用不同。 |
| 关键词: 苦皮藤素V 细胞色素P450 NADPH－细胞色素P450还原酶 选择毒性 |
| DOI： |
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| 基金项目:国家自然科学基金资助项目(30130130) |
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| Studies on the relationship of cytochrome P450 and selective toxicity of Celangulin Ⅴagainst insect |
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| Abstract: |
| This paper discussed the relationship of cytochrome P450 content and NADPH-cytochrome P450 reductase activity with the selective toxicity of celangulin Ⅴ against Mythimna separata and Agrotisypsilon.In normal groups,the cytochrome P450 content and the NADPH-cytochrome P450 reductase activity of A.ypsilom and M.separata were significantly higher than that of control;P450 content of M.separata increased 1.46 and 2.26 fold respectively in the period of convulsion and the period of losing body fluid,and NADPH-cytochrome P450 reductase activity increased 1.26 and 2.56 fold respectively than that of normal.P450 content of A.ypsilon treated with celangulin Ⅴ increased 0.39 fold and NADPH-cytochrome P450 reductase activity increased slightly.These results indicated the content of cytochrome P450 and the activity of NADPH-cytochrome P450 reductase have important relationship with the selective toxicity of Celangulin Ⅴ against Mythimna separata and Agrotis ypsilon larvas,meanwhile,celangulin Ⅴ has certain inductive function to insect cytochrome P450,but the inductive function is varied to different insects and different composition of cytochrome P450 of an insect. |
| Key words: Celangulin Ⅴ cytochrome P450 NADPH-cytochrome P450 reductase selective toxicity |
