| 摘要: |
| 为进一步研究基因疫苗的免疫机制和基因疫苗的免疫效果，应用DNA重组技术，根据载体pcDNA4.0/His Max的多克隆位点设计引物，对含有NDV保护性抗原基因F的质粒PCR扩增。将得到的目的片段插入到载体的启动子下游，成功得到含有NDVF基因的真核表达质粒载体PC4F的基因疫苗。 |
| 关键词: 鸡新城疫病毒 F基因 基因疫苗 |
| DOI： |
| 分类号: |
| 基金项目:国家“九五”科技攻关项目(96-C01-04-01) |
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| Construction of highly expressed eukaryotic plasmid containing NDV F gene |
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| Abstract: |
| The recombinant plasmid PC4F that contains the gene of protective antigen F of NDV was constructed by method of the PCR and restricted enzyme.The F geng was inserted into the downstream promotor of pcDNA4.0/His Max vetor.The result shows that we successfully get a DNA vaccine of NDV F gene that can be hightly expressed in the eukaryotic cell in order to study the mechanism and effect of the DNA vaccine. |
| Key words: chicken newcastle disease virus F gene DNA vaccine |
