| 摘要: |
| 论述了和分析了影响苹果高效离体再生的主要因素，包括试验材料苹果的基因型，外植体生理状态，培养基类型，植物激素，AgNO3，前期暗培养，抗生素等内外因子，以及影响根癌农杆菌介导苹果遗传转化的主要因素，包括苹果基因型，外植体生理状态，菌株类型，菌液浓度，活化物质的应用，共培养，预培养，选择培养，培养基成分等内外因子，并对其研究现状，存在的问题及应用前景作了简要概述。 |
| 关键词: 苹果 根癌农杆菌 遗传转化 影响因子 |
| DOI： |
| 分类号: |
| 基金项目:国家自然科学基金资助项目(39970522) |
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| Advance of research on genetic transformation mediated by Agrobacterium tumefaciens in apple |
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| Abstract: |
| The paper summarizes and analyses main influencing factors of highly efficient regenerations in vitro including apple genotype,explants properties,plant hormone ratio and level,AgNO3,dark incubation,antibiotics,et al,and influencing factors of transformations mediated by Agrobacterium tumefaciens including apple genotypes,explants properties Agrobacterium strain,Agrobacterium concentration,function of activated matters co cultivation,pretreatment,selection culture,medium types,et al.The present situation of its studies,existing problems and its prospects are briefly mentioned. |
| Key words: apple Agrobacterium tumefaciens genetic transformation influencing factors |
