| 摘要: |
| 为了解决硝酸铝比色法在测定杜仲总黄酮时含量偏高的问题，用外标法和纸层析法对干扰测定的因素和测定方法进行了研究。结果表明，绿原酸是硝酸铝比色法测定杜仲黄酮的主要干扰因素，干扰程度大于50％；将纸色谱与硝酸铝比色法结合进行测定，可消除干扰，准确度提高，标准差为10.6 mg/g，测定回收率为99.75％。 |
| 关键词: 杜仲叶 总黄酮测定 绿原酸 |
| DOI： |
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| 基金项目:陕西省科技研究重大项目(97K04-G2) |
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| A study on the measurement of flavonoids in the leaves of Eucommia ulmoides |
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| Abstract: |
| It was found that when aluminum nitrate was used as a chromogenic agent in the colorimetric measurement of total flavonoids in the leaves of Eucommia ulmoides,the result was deeply interfered by chlorogenic acid,a main chemical contained in the leaves,which possessed a structure of o-phenolic di-hydroxyls.Satisfactory result will be obtained if the paper chromatography is integrated with colorimetric measurement. |
| Key words: Eucommia ulmoides leaf measurement of flavonoids chlorogenic acid |
