| 摘要: |
| 提取、分离了雷公藤（Tripterygium wilfordii Hook）根皮中非生物碱物质，经鉴定其主要成分为总萜内酯，含量为105.3 g/kg。以粘虫为试虫，对非生物碱物质杀虫活性和作用方式进行了系统的生物测定。结果表明，非生物碱对粘虫具有拒食、毒杀、麻醉、触杀和杀卵作用。拒食中质量浓度AFC50为50.26 mg/L；毒杀中质量浓度LC50为34.72 mg/L，麻醉中量ND50为13.45 μg/g，触杀中量LD50为3.54 μg/头，在非生物碱质量浓度为0.5～20 g/L时，34％～65％的粘虫卵不能孵化。 |
| 关键词: 雷公藤 非生物碱 粘虫 杀虫活性 |
| DOI： |
| 分类号: |
| 基金项目:高等学校博士点基金(003518);杨凌农业高新技术产业示范区重大科技开发基金(98A19) |
|
| Isolation and bioactivities of the non-alkaloids from Tripterygium wilfordii against Leucaninia separata Walker |
|
|
| Abstract: |
| The non-alkaloids were extracted from the root bark of Tripterygium wilfordii Hook and identified.The results of bioassay showed that the non-alkaloids have obviously insecticidal activities to Leucania separata Walker.LC50 value of poisoning to the 5 th instarts of Leucania separata,is 34.72 mg/L.The value of medium antifeeding concentration(AFC50) of the non-alkaloids extracts against the 5 th larval of Leucania separata is 50.25 mg/L.Median narcosis dose (ND50) is 13.45 μg/g.Median contact dose (LD50) is 3.54 μg/head.When the concentration of the non-alkaloids is 0.5-20 g/L,the unhatching of eggs ratio is 34%-60%. |
| Key words: Tripterygium wilfordii Hook,Non-alkaloids,Leucania separata Walker insecticidal activity |
