| 摘要: |
| 研究了3种外源激素对3个小麦品种(系)幼胚体细胞胚性无性系高频率形成的影响。结果表明2,4-D能显著促进小麦幼胚体细胞胚性无性系的形成，并存在着基因型间的差异，在小麦体细胞无性系形成中起主要作用。其最佳适用质量浓度为2.0～3.0 mg/L，2,4-D的这种作用能被培养基中加入的细胞分裂素所加强，这种作用在不同的细胞分裂素间是有差异的，在质量浓度0.1～2.0 mg。 |
| 关键词: 小麦 外源激素 幼胚 胚性无性系 |
| DOI： |
| 分类号:S814.8 |
| 基金项目:陕西省科技攻关项目(2000K07-G1-01)；杨陵生物技术育种中心资助项目(99-8) |
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| Effect of exogenous hormones on high frequency induction of embryogenic somaclone derived from immature embryos of wheat (Triticum aestivum L.) |
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HUA Jin lian,DOU Zhong ying,LI Song,LEI An min,YANG Chun rong,HUA Jin lian,DOU Zhong ying,LI Song,LEI An min,YANG Chun rong,HUA Jin lian,DOU Zhong ying,LI Song,LEI An min,YANG Chun rong
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| Abstract: |
| Effect of three exogenous hormones on high frequency induction of three varieties of wheat (Triticum aestivum L.) immature embryos is studied.The following results are acquired.Concentration of 2,4-D in media significantly affects the induction of embryogenic calli derived from immature embryos of wheat,with different frequency between the three genotypes.The most appropriate concentration of 2,4-Dis at 2.0-3.0 mg/L.This capability of 2,4-D can be enforced by the addition of KT or 6-BA in medium.But this enforcement differs between KT and 6-BA.KT has a significant role in the induction of embryogenic calli at relatively high concentration while 6-BA works well at lower concentration. |
| Key words: wheat (Triticum aestivumL.) exogenous hormorne immature embryo embryogenic somaclone |
