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中国野生葡萄抗白粉病基因的RAPD标记
王跃进1, 张剑侠1, 周 鹏2
1.西北农林科技大学 园艺学院;2.热带作物生物技术国农重点实验室
摘要:
利用随机扩增多态性DNA(RAPD)技术,对抗病×感病的葡萄种间杂交组合白河-35-1×佳利酿的亲本及其F1代的24株杂种进行了抗白粉病的遗传分析,从196个随机引物中获得了1个与中国野生葡萄抗白粉病基因连锁的RAPD标记OPV03-1380,并在中国野生葡萄华东株系和欧洲葡萄品种中进行了分析验证。
关键词:  中国野生葡萄  白粉病  抗病基因  RAPD
DOI:
分类号:Q969.36 4.2
基金项目:国家自然科学基金资助项目(39770525)、高校博士点基金(980701)和教育部资助年青优秀教师基金项目
RAPD marker of resistance gene to Uncinula necator in Chinese wild Vitis
WANG Ying lun,CHOU Io,YUAN Feng,WANG Ying lun,CHOU Io,YUAN Feng,WANG Ying lun,CHOU Io,YUAN Feng
Abstract:
The gentic analysis of the resitance to powdery midew of Chnese wild Vitis was carried out with the parents and 24 F1 individuals of interspecific hybridization combination Baihe-35-1×Carignane (Resistant×Susceptible) by randomly Amplified Polymorphic DNA (RAPD) technique.A total of 196 random primers were screened on the DNA of resistnat and susceptible materials.OPV03-1380,a PAPD marker,which linked to powdery mildew resistant gene in Chinese wild Vitis,was obtained.Additionally,the OPV03-1380 was tested among clones of V.pseudoreticulata of Chinese wild Vitis and varieties of V.vinifera.
Key words:  Chinese wild Vitis  powdery mildew  resistance gene  RAPD

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