| 摘要: |
| 运用SDS-PAGE和PCR分析了小偃6号及其亲本中高分子质量麦谷蛋白亚基(HMW-GS)组成。结果表明,小偃6号的2个亲本ST2424 /464和小偃96中都存在14和15亚基,而远缘种长穗偃麦草中不存在14和15亚基。从而证明长穗偃麦草并非小偃6号14和15亚基的来源,而ST2424/464和小偃96均有可能是14和15亚基的提供者。通过PCR分析发现了偃麦草中1个不同于普通小麦的麦谷蛋白基因。 |
| 关键词: 小麦,高分子质量麦谷蛋白,SDS-PAGE,PCR |
| DOI： |
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| 基金项目:国家农业生物技术开放实验室资助项目(HMW-GS14和15研究) |
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| A study on the origin of HMW-GS 14 and 15 in Xiaoyan 6 |
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| Abstract: |
| Through studying HMW-GS composition of Xiaoyan 6 and its parents by SDS-PAGE and PCR, it is found that its two parents ST2424/464 and Xiaoyan 96 have subunits 14 and 15.Although Some researchers believe elongate Elytrigia is the origin of them, this study show it is not a right conclusion.Using PCR,we get a specific fragment of HMW-GS in Elytrgia. |
| Key words: wheat HMW-GS SDS-PAGE PCR |
