| 摘要: |
| 对平邑甜茶叶盘离体再生条件进行了研究,结果表明,平邑甜茶叶盘愈伤和分化适宜的BA和IBA质量浓度分别为0.5～1.0和0.2mg/L,愈伤至少需黑暗培养35d;茎尖分化的适宜培养基为MS附加BA0.5mg/L、IBA0.1～0.2 mg/L;适宜的生根培养基为1/2MS附加IBA0.1mg/L。 |
| 关键词: 平邑甜茶,组织培养,植株再生,叶盘法 |
| DOI： |
| 分类号:S571.103.53 |
| 基金项目:西北农业大学青年教师科研基金资助项目 |
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| Study on the regeneration of Malus hupehensis Rehd. |
|
ZHANGJun-ke,ZHANGJun-ke,ZHANGJun-ke
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| Abstract: |
| Conditions influencing regeneration by leaf disk of Pingyitiancha (Malus hupehensis hd.) were studied.The result showed that the optimum concentrations of benzyladenine (BA) and Indole-3 butyric (IBA) for Pingyitiancha leaf disk callus inducing and differentiation were 0.5-1.0 mg/L and 0.2 mg/L respectively.35-days dark culture was needed during callus inducing.The optimum medium for shoot tip differentiation was MS supplemented with BA 0.5 mg/L and IBA 0.1-0.2 mg/L.The optimum medium for rooting was 1/2MS supplemented with IBA 0.1 mg/L. |
| Key words: Malus hupehensis Rehd.,tissue culture,plant regeneration,leaf disk method |
