| 摘要: |
| 从二棱大麦 (Hordeum distichon Linn.)幼苗中提取总 RNA,通过 RT- PCR获得目的片段 HDCS1 ,并采用 T-载体进行克隆。测序结果表明 ,HDCS1全长 664bp,含有 1个完整的阅读框架。其编码蛋白质全长 2 0 2个氨基酸 ,含有 8个由 1 1个氨基酸残基组成的基元序列 ,属第 3组 LEA蛋白。HDCS1与大麦 (Hordeum vulgare L.) LEA蛋白、HVA1具有高度同源性 ,其差异在于 HDCS1比 HVA1缺少一个完整的由 1 1个氨基酸残基组成的基元序列 |
| 关键词: 二棱大麦,LEA基因,克隆 |
| DOI： |
| 分类号:S188 |
| 基金项目:杨陵农业科技开发基金资助项目(4130202) |
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| Cloning of a group 3 Lea cDNA from tworow barley |
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RAO Jing-ping,RAO Jing-ping,RAO Jing-ping
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| Abstract: |
| Total RNA is extracted from tworow barley (Hordeum distichon Linn.) seedlings induced with 0.1 mmol/L ABA and reversely transcribed to first strands of cDNA.The interest fragment,HDCS1,is obtained by PCR and cloned by T-overhang vectors.HDCS1 is 664 bp long containing one open reading frame.It encodes a deduced protein of 202 amino acid residues,which contains eight motifs composed of 11 amino acids and belongs to group 3 LEA protein.HDCS1 is highly homologous to barley (H.vulgare Linn.) HVA1.The only difference between them is that the number of 11-mer repeating units of HDCS1 is one less than that of HVA1. |
| Key words: tworow barley,LEA cDNA,cloning |
