| 摘要: |
| 从萆薢中分离出一种白色针状结晶,经TLC和MP鉴定为薯蓣皂甙。萆薢粉经乙醇提取后制成水剂,比色法测定薯蓣皂甙质量浓度为4. 785 7 g·L-1.将30只痛风鸡随机分成3组,每组10只,第Ⅰ组为对照;第Ⅱ组每日用肾肿清按10 g·kg-1的比例拌料饲喂;第Ⅲ组每日饮水中添加52. 5mL皂甙水剂。每间隔3 d颈静脉采血3～5mL /只,分别测定血液尿酸、血清钙、血清磷的含量。结果表明,第Ⅱ,Ⅲ组血液尿酸含量比第Ⅰ组明显降低,差异显著(P<0. 05);第Ⅱ,Ⅲ组血清钙和血清磷含量与第Ⅰ组差异不显著(P>0. 05);第Ⅱ组血液尿酸、血清钙和血清磷含量与第Ⅲ组差异不显著(P>0. 05)。第Ⅱ组和第Ⅲ组治愈率分别为60%和80%。 |
| 关键词: 萆薢,鸡痛风,尿酸,薯蓣皂甙 |
| DOI： |
| 分类号:S858.313.73 |
| 基金项目:西北农业大学青年基金资助项目 |
|
| Isolation of the dioscin from rhizoma dioscoreaecolletii and its effect on gout |
|
|
| Abstract: |
| Dioscin, a white needle crystal isolated from Rhizoma Dioscoreae colletii,
was identified by TLC and MP.The powder of Rhizoma Dioscoreae colletii was extracted
with 800mL /L ethanol to prepare water agent of dioscin with the content of dioscin being 4. 785 7 g·L-1. 30 chicks were divided into 3 groups randomly, 10 chicks in each
group.GroupⅠ was control,GroupⅡ was fed with forage containing 1 percent Shenzhongqing each day and GroupⅢ added 52. 5mL prepared water agent in drink water
each day.Blood samples (3～5mL /chick) were collected from jugular vein with an interval of 3 days. The contents of uric acid, serum calcium and phosphorus were determined.The results revealed that the contents of uric acid in groupsⅡ and Ⅲ were significantly lower than GroupⅠ(P< 0. 05), but there was no significant difference between groupsⅡ and Ⅲ(P>0. 05).The contents of serum calcium and phosphorus were
noticed in GroupⅡ. There was no significant difference among three groups (P>0. 05).The cure rates of groupⅡ and Ⅲ were 60 percent and 80 percent, respectively. |
| Key words: rhizoma dioscoreae colletii,chicken gout,uric acid,dioscin |
