| 摘要: |
| 研究了葡萄糖氧化酶(GOD)、β-D-葡萄糖(β-D-G)、辣根过氧化物酶(HRP)、色原物(CS)及pH值等因素对放大系统的影响。结果表明,放大系统的最佳pH值为6. 4,理想色原物是联大茴香胺, 3, 3′, 5, 5′-四甲基联苯胺(TMB)和邻苯二胺(OPD)不宜作为放大系统的色原物。GOD,β-D-G,HRP和CS在不同程度上均极显著(P<0. 01)地影响试验结果,其主次关系为GOD>β-D-G>>HRP>CS.反应时间对试验结果有显著(P<0. 05)影响。适当增加反应物浓度和延长反应时间对放大系统是有利的。 |
| 关键词: 信号放大系统,连串反应,ARIS |
| DOI： |
| 分类号:Q554.6 |
| 基金项目:高等学校博士点基金(4397)，陕西省杨陵科研基金(06B5) |
|
| Study on signal amplification system of ARIS |
|
|
| Abstract: |
| Influences of glucose oxidase (GOD),β-D-glucose (β-D-G ), horseradish
peroxidase (HRP), chromogeneous substance (CS) and pH value on SAS were studied in
this paper.The results show that the o-ptimum pH value for SAS is 6. 4 and odianisidine
(ODA ) as CS fits the SAS of ARIS, but 3, 3′, 5, 5′-tetramethylbenzidine and ophenylene diamine not.GOD,β-D-G,HRP and CS have significant effects on the SAS on
different degrees respectively (P<0. 01). Their important order is GOD>β-D-G>>
ODA >HRP. Reaction time also has significant influence on the SAS (P< 0. 05).
Increasing reactant concentration and prolonging reaction time benefit the SAS. |
| Key words: signal amplification system,continuous reaction,ARIS |
