| 摘要: |
| 机械方法处理胎牛、犊牛和成年牛卵巢可以获得大量的腔前卵泡。胎牛卵巢用眼科手术剪刀剪碎(M1)或用组织切碎机切碎(M2),犊牛和成年牛卵巢用组织切碎机切碎,经悬浮吹打和过滤,平均每只卵巢可获得腔前卵泡胎牛为3 282(M1)和5 688(M2),犊牛为2 100,成年牛为352.在所分离的胎牛和犊牛卵巢卵泡中原始卵泡和初级卵泡占绝大部分,而成年牛卵巢卵泡主要是初级卵泡和次级卵泡。不论是胎牛、犊牛或成年牛,从其所分离的绝大部分腔前卵泡外观质量完好。 |
| 关键词: 牛卵巢，腔前卵泡，机械分离 |
| DOI： |
| 分类号:S823.811,S814.1 |
| 基金项目:农业部生物技术重点项目 |
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| Mechanical Isolation of Preantral Follicles in Bovines |
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| Abstract: |
| A large number of preantral follicles had been obtained by mechanical
procedure from bovine fetuses, calves and cows. The ovaries of fetuses were cut into
small pieces (≤1mm) with both an optical scissors (M 1) and a tissue chopper (M 2),
and ovaries of calves and cows were cut with a tissue chopper.Then the suspensions of
ovarian fragments were suspended and filtered.The mean numbers of preantral follicles
isolated from each ovary of fetuses, calves and cowswere 3282 (M 1) and 5688 (M 2),
2100 and 352 respectively.The vast majority of the ovarian follicles isolated from fetus
and calf ovaries consisted of primordial follicles and primary follicles, and mainly primary and secondary follicles from cow ovaries,most of which had good quality. |
| Key words: bovine ovary,preantral follicle,mechanical isolation |
