| 摘要: |
| 选用杜洛克2～16细胞期胚胎卵裂球作核供体,湖北白猪卵母细胞作核受体,通过显微操作和电融合法构成重组胚。体外培养时,以融合前2 h,1 h激活及融合前不激活的卵母细胞做核受体的重组胚,发育率分别为64.6%(31/48),55.3%(26/47)和34.5%(19/55)。61枚重组胚移入同步发情的5头受体母猪输卵管,1头于妊娠117 d产下5头核移植仔猪。结果表明,激活卵母细胞作核受体优于未激活卵母细胞。成熟卵母细胞的胞质对于移入的核具有重排能力。 |
| 关键词: 猪胚胎，核移植，电融合，电激活 |
| DOI： |
| 分类号:S827.3 |
| 基金项目:国家自然科学基金和湖北省自然科学基金资助项目 |
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| Nuclear Transplantation in Porcine Embryos |
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Chen Naiqing 1 Zhao Haobin 2 Gou Deming 1 Fan Junhua 2,Chen Naiqing 1 Zhao Haobin 2 Gou Deming 1 Fan Junhua 2,Chen Naiqing 1 Zhao Haobin 2 Gou Deming 1 Fan Junhua 2
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| Abstract: |
| Durac was used as nuclear donor and Hubei White used as nuclear recipient.Reconstructive embryos were produced with electrofused method by micromanuplantation.Thedevelopmentrateofnuclear transfer embryos (NTE) was 64.6%,55.3% and 34.5% (P<0.05) respectively,when the recipient oocytes were activated two hours,one hour prior to fusion and nonactivated.61 nuclear transferred reconstructive embryos were transferred to five recipient sows.One recipient calved five nuclear transfer piglets.The resultes indicated that activated oocytes could be used as recipient and could remodel the transferred nucleus.The birth of NT piglets showed that the technical parameters and procedures in this paper were suitable for nuclear transplantation in porcine embryos. |
| Key words: porcine embryo,nuclear transplantation,electrofusion,electroactivation |
