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快生型大豆根瘤菌生物学特性研究
程丽娟1, 刘海轮1
西北农业大学 农化系
摘要:
由当地分离纯化并回接有效而获得的4株快生型大豆根瘤菌株,通过形态、生理鉴定,表明与慢生型菌株有明显差异;在菌株与大豆品种亲和性的盆栽试验中,其中A034菌株与“徐州四号”、A034与“东解选”大豆品种间具有良好共生效应;其株高、茎叶干重及含氮量、株瘤数与瘤重、及固氮酶活性等,均明显高于不接种对照;固氮率分别提高为48.1%与44.5%;
关键词:  快生型大豆根瘤菌,菌株特性,共生效应,固氮率
DOI:
分类号:S565.101
基金项目:
Biological Characteristics of Four Fast-Growing Rhizobium japonicum Strains
Cheng Lijuan Liu Hailun,Cheng Lijuan Liu Hailun
Abstract:
Four fast-growhg Rhizobium japonicum strains were isolated,purified and back-nodulated to soybeans in the local areas.The results from the identification of morphology and physiology showed that there were obvious differences between these strains and slow-growing Rhizobium japonicum strains.In the pot experiments of an ffinity between the strains and soybean cultivars,the excellent symbiotic effect was found among strain A034 and soybean cultivar-Xuzhou NO.4 and strain A032 and soybean cultivar-Dong Xiexuan,whose plant height,dry weight and nitrogen contents of stems and leaves,and also activity of nitrogenase were higher than those of the control soybean plants(without inoculation).Nitrogen-fixing efficiency was increased by 48.1% and 44.5% respectively.
Key words:  fast-growing Rhizobium japonicum,strain characteristics,symbiotic effect,nitrogen-fixing efficiency

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