| 摘要: |
| 完成了弗兰克氏菌菌株C101,Cc01,CcR03,At4,Hr16和CG01的原生质体分离及Cc01原生质体的再生。将菌丝转化技术用于弗兰克氏菌的转化研究,结果发现,pLAFRI上的四环素抗性基因能在Cc01中表达。此外,Cc01具有卡那霉素抗性。 |
| 关键词: 原生质体,分离,再生(细胞),基因表达,放线菌/弗兰克氏菌,转化 |
| DOI： |
| 分类号: |
| 基金项目:国家自然科学基金资助项目 |
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| A Preliminary Study on Transformation of Frankia |
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Zeng Wangyong Bai Xueliang Ma Qingsheng,Zeng Wangyong Bai Xueliang Ma Qingsheng,Zeng Wangyong Bai Xueliang Ma Qingsheng
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| Abstract: |
| Protoplast formation of Frankia stain C101, Cc01, CcR03, At4, Hrl6 and CG01, and protoplast regeneration of Cc01 were achieved. Transformation of hyphae was used in transformation of Frankia, and it was found that tetracyclin resistance gene can express in strain Cc01. In addition, stain Cc01 confers Kanamycin resistance. |
| Key words: protoplast, separation, regeneration(cytology), gene expression, Actinomycetes/Frankia, transformation |
