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猪血清中游离CD2对红细胞的溶解作用
肖俊杰1, 陈德坤1
西北农业大学动物病毒病研究所
摘要:
猪血清中加入红细胞(RBC)后观察溶血的结果表明,正常猪血清对绵羊、山羊、牛、马、家兔、豚鼠及鸡RBC的溶血指数分别为72.10±22.84%,84.87±22.26%,7.66±4.77%,66.60±19.56%,16.40±7.40%,86.59±15.29%,88.45±26.76%;对同种RBC无溶解作用或具微弱的溶解作用。根据猪血清对RBC的溶解作用,经绵羊红细胞(SRBC)吸收或加入SRBC CD58(淋巴细胞功能相关抗原-3,LFA-3)竞争结合后即显著降低或消失,提出了猪血清溶解RBC的作用与血清中游离CD2的存在有关的论断。
关键词:  溶血,红细胞,血清学,免疫生物学,猪/游离CD2
DOI:
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基金项目:
Effects of Free-Form CD2 in Pig Serum Upon RBC
Xiao Junjie Chen Dekun,Xiao Junjie Chen Dekun
Abstract:
The observation was made after RBC was added to pig serum ,The results showed that the haemolysis index of normal pig serum upon RBC of sheep,goat,cattle,horse,rabbit.guinea-pig and chicken is 72.l0±22.84%;84.87±22.26%;7.66±4.77%;66.60±l9.56%;l6.40±7.40%;86.59±15.29%;and 88.45±26.76% respectively.There is no or only a 1ittle distructive effect upon allogenic RBC.This distructive effect of pig serum on RBC will decrease significantly disappearing after serum was absorbed with SRBC or added SRBC,CD58(LFA-3)in it.Thus,the related deduction of distructive effect of pig serum upon RBC and the presence of free-CD2 in pig serum was made.
Key words:  hemolysis, erythrocytes, serology, immunobiology, pigs/free CD2

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