| 摘要: |
| 选择小鼠晚期桑椹胚和早期囊胚,采用下列方法进行分割:A、显微手术刀直接分割;B、酶消化透明带后分割;C、显微玻璃针去带分割;D 酶——机械去带分割。共分割277枚胚胎,分割成功264枚(95.3%)。将其中480枚半胚作体外培养,A,B,C,D 各组半胚发育到囊胚的发育率分别为65.7%(92/140)、54.2%(65/120)、63(63/100)、64.2%(77/120)。将用 A法分割的48枚半胚移植于12只受体鼠,结果有4只怀孕,共产11只半胚鼠,其中4对有同卵双生仔鼠。 |
| 关键词: 胚胎分割,半胚,同卵双生,小鼠 |
| DOI： |
| 分类号: |
| 基金项目:国家自然科学基金“家畜胚胎移植研究”的一部分 |
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| PROCEDURES OF BISECTING EMBRYOS AND PRODUCTION OF MONOZYGOTIC TWINS IN MOUSE |
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Zhang Yong Qian Jufen Wang Jianchen,Zhang Yong Qian Jufen Wang Jianchen,Zhang Yong Qian Jufen Wang Jianchen
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| Abstract: |
| Late morulae and early blastocysts were selected to carry out the embryos bisection with the following procedures:A.by a microblade directly;B.by a microblade after removal of the zonae pellucide by digestion with pronase; C.by a fine glass needle after mechnical removal of the zonae pellucide;D. by a microblade after enzymic and mechnical removal of the zonae pellucide. Of 277 embryos,264(95.3%)were successfully bisected by the above procedures.480 thalf embryos were cnltured in vitro.The development rate of them to blastocysts in A,B,C,and D groups was 65.7%(92/140);54.2% (65/120);63%(63/100)and 64.2%(77/120)respectively.48 half embryos bisected by the microblade were transfered to 12 pseudopregnant recipients,of which 4 became pregnant.11 live young were obtained at delivery with 4 pairs of monozygotic twins included. |
| Key words: bisection of embryos,half embryos,monozygotic twins,mouse, |
