| 摘要: |
| 应用4～10周令的杂种 CB6/Han 和纯种 C57BL/Han 小鼠73只进行试验,共采胚胎523枚,将发育正常,形态完好的157枚胚胎低温冷冻保存。解冻后回收率为83.4％,其中45％(59枚)形态完好,(囊胚56％,桑椹胚20％)。将其培养24—48小时后,71.2％(42枚)继续发育到扩张囊胚(其中囊胚为76.5％,桑椹胚为37.5％)。应用3′6′—二乙酰基荧光素(FDA)染色进行胚胎质量评定,对胚胎无损害。 |
| 关键词: 小鼠,胚胎,冷冻,囊胚,桑椹胚,3’6’—二乙酰基荧光素 |
| DOI： |
| 分类号: |
| 基金项目: |
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| Experiment on the Preservation of Mouse Embryos by Freezing |
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Dou Zhongying Northwestern Agricultural University
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| Abstract: |
| The experiment was conducted by using 73 mice of hybrid CB6/Han and pure bred C57BL/Han,aged 4-10 weeks,from which 523 embryos were collected.There were 157 normally developed and intact embryos to be preserved in freezing conditions.After unfreezing,the recovery percentage was 83.4％，of which 45％ of embryos (59) remained intact (56％ were blastocyst,and 20％ morula).After 24-48 hours of culture,these embryos continued their development into expanding blastoysts,with the morulal being 37.5％,and the blastocyst 76.5％.3'6'-Fluorescein-Diacetyl (FDA) was used in staining,and no damage to the embryos was found. |
| Key words: Mouse,embryo,freezing,blastocyst,morula,3'6'-Fluorescein-Diacetyl (FDA) |
