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Musclin基因编码蛋白的生物信息学分析
孙超,王力,齐仁立,姜东凤
作者单位
孙超 西北农林科技大学 动物科技学院陕西 杨凌 712100 
王力  
齐仁立  
姜东凤  
摘要:
【目的】 克隆猪Musclin基因,分析和预测其编码蛋白的结构与功能。【方法】 从猪肌肉组织中提取总RNA,RT-PCR扩增获得Musclin基因,对其进行克隆和测序,并根据生物信息学分析方法,利用Tmpred、SignalP3.0、TargetP 1.1等分析软件,对猪Musclin蛋白的跨膜结构、信号肽、细胞定位、结构特征等进行分析和预测。【结果】 猪Musclin蛋白主要集中在分泌途径上,存在于细胞外,其核苷酸序列与家兔的具有较高的相似性,氨基酸序列32~45位和55~63位存在2个可能的强跨膜螺旋区,该蛋白的空间结构以α螺旋、β转角和无规则卷曲为主;其1~26位氨基酸序列是一段信号肽,在26与27位氨基酸之间存在1个酶切位点;其羧基端含有较多的转角结构,同时该区域的抗原指数较高,亲水性指数和呈现在蛋白表面的可能性均较大。【结论】 Musclin是一种含有信号肽序列的跨膜蛋白。
关键词:  Musclin  生物信息学  结构与功能  序列分析
DOI:
分类号:
基金项目:国家自然科学基金项目(30871785);教育部重点项目(105167);教育部新世纪优秀人才计划项目(NCET-06-0865);国家“十一五”科技支撑计划项目(2006BAD04A11)
Analysis of the structure and function of porcine Musclin protein by bioinformatics
SUN Chao,WANG Li,QI Ren-li,JIANG Dong-feng
Abstract:
【Objective】 This study was to clone the gene of porcine Musclin protein as well as analyze and predict structures of this protein.【Method】 Total RNA was extracted from porcine muscle tissue.Reverse transcription-polymerase chain reaction (RT-PCR) was used to detect Musclin gene.According to the analysis methods of bioinformatics,the research applied many analysis programs,e.g.Tmpred,SignalP 3.0,TargetP 1.1 to analyze and predict the structural characteristics of porcine Musclin protein,its transmembrane helices and signal peptide.【Result】 Most of Musclin exist in extracellular space.There is a high similarity of Musclin nucleotide sequence between swine and rabbit,and the main structures of Musclin protein are α-spirals,β-turn and random coil.1-26 amino acid is a signal peptide and there is a enzyme cutting site between 26 and 27.Moreover,β-turn is abundant in the C-terminal region,and this domain is supposed to be the epitopes of the B cell.This results would be helpful for the research of immunological function of Musclin.【Conclusion】 Musclin is a transmembrane protein which comprises signal peptides.
Key words:  Musclin  bioinformatic  structure and function  sequence analysis