| 摘要: |
| 用合适的表面活性剂、VE油溶液和VC水溶液制备一种适合皮肤涂抹用药的VC和VE复方微乳，并用透射电子显微镜测定其粒径，高速离心法、温度梯度法和恒温加速试验检验其稳定性，紫外分光光度法测其有效成分含量，急性毒性试验、皮肤刺激性试验、眼刺激性试验检验其安全性。结果表明，制备的VC和VE复方微乳粒径为10～100 nm，符合微乳的粒径要求。样品经4 000 r/min离心30 min和37 ℃相对湿度为75%的条件下保存3个月均保持澄清透明，未见分层或絮凝，其稳定温度范围为4～60 ℃。样品中VC和VE的含量分别为(182.91±2.39)和(13.97±0.60) mg/mL,平均回收率分别为103.3%和96.7%。该VC和VE复方微乳无经口毒性、皮肤刺激性和眼刺激性，适合用于皮肤给药。 |
| 关键词: VC与VE复方微乳 微乳稳定性 微乳安全性 |
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| 基金项目:西北农林科技大学校长专项基金项目（130709） |
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| Study on the preparation and quality evaluation of vitamin C and vitamin E combination micro emulsion |
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| Abstract: |
| To prepare a vitamin C and vitamin E combination micro-emulsion with suitable surfactant,vitamin E oily solution and vitamin C aqueous solution and evaluate its average diameter,physical stability,containing concentration of vitamin C or vitamin E and Safety by electron microscope,stability tests,uv spectrophotometry,and safety were tested respectively.The results showed that the average diameter of the sample was 10-100 nm,which accorded with that of micro emulsion.It was stable after going through centrifugation at 4 000 rpm for 30 min and storage at 37 ℃ for three months,the range of the stable temperature was 4-60 ℃.The accuracy of assaying was good.The results of the safety evaluations showed that the micro-emulsion had no toxicity and stimulation to the skin and eyes.So，vitamin C and vitamin E combination micro-emulsion is suitable for skin drug delivery systems. |
| Key words: Vitamin C Vitamin E micro-emulsion stability safety |
