| 摘要: |
| 用高效液相色谱仪测定了盐酸黄连素在4种油(IPM、大豆油、花生油、色拉油)和3种表面活性剂(EL40、大豆卵磷脂和Tween-80)中的溶解度；根据纳米微乳的评价标准，利用序贯设计思想，采用滴定法绘制了伪三元相图，确定了纳米微乳的最佳组成,研制了盐酸黄连素纳米微乳，并对其基本理化特性（形态、粒度、颜色、澄清度、稳定性和自乳化速度等）及体外抗菌活性进行了研究。结果显示，盐酸黄连素纳米微乳三相最佳组成是EL40-甘油-IPM；制得的纳米微乳为澄清透明的球状液滴，其平均粒径为56.8 nm；高速离心、高温、强光下无分层，也无絮凝或药物析出；与传统片剂、胶囊和水溶液对大肠杆菌、沙门氏菌、金黄色葡萄球菌和无乳链球菌的抗菌活性相比，纳米微乳的抗菌活性更高。以上研究表明制备的盐酸黄连素纳米微乳是一种质量稳定、高效的抗菌剂。 |
| 关键词: 黄连素 纳米 微乳 稳定性 抗菌活性 |
| DOI： |
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| 基金项目:西北农林科技大学校长基金项目（130709） |
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| The preparation and antibacterial activity in vitro of berberine hydrochloric nanometer microemulsion |
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| Abstract: |
| The solubility of berberine hydrochloric respectively dissolved in four kinds of oil (IPM,soybean oil,peanut oil,salad oil) and three kinds of SF EL40,soybean phosphatidylcholine,Tween-80 were measured by HPLC.The composition was preliminarily determined using the key evolutional normal of nanometer microemulsion.The pseudotertiary phase diagrams were protraited by titrating,according to the sequential design theory.The formulations of the berberine nanoemulsion were optimized.The basic physicochemical characteristics including the morphology,diameter,color,defecation and the self emulsifing speed and stability were studied.The antibacterial pharmacodynamics of the nanometer microemulsion was studied in vitro.In this test,the best composition (EL40-Glycerol-IPM) was determined.The nanometer microemulsion was a kind of clear solution with small spherical drops under election microscopy with an average diameter of about 56.8 nm.The nanometer microemulsion was stable under high temperature and strong light conditions.Compared with the berberine hydrochloric tablet,capsule,watery solution,blanking nanometer microemulsion to S.aureas,E.coli,S.agalactae,Sam onellal,berberine hydrochloric nanometer microemulsion had stronger antibacterial action than other dosage-forms.The results show that the nanometer microemulsion is the good antibacterial preparation with stable quality and high efficiency. |
| Key words: berberine hydrochloric nanometer microemulsion stability antibacterial activity |
