| 摘要: |
| 应用RAPD技术对秦川牛及其杂种牛进行了遗传多样性和屠宰性能（宰前重、胴体重及净肉重）的遗传标记研究，结果表明：①F09和CY0814两条引物共扩增出13条DNA条带，平均多型条带频率0.692，其中F09引物的多型条带频率为0.571 4，CY0814引物的多型条带频率为0.833 3。②对肉用性能RAPD标记时，CY0814引物D带的1型与0型对宰前重的效应值离差为22.17 kg；F09引物的A带0型与1型对胴体重和净肉重的效应值离差分别为14.32和11.36 kg。③在多条带组合效应分析中，CY0814引物的ABCD组合对宰前重的效应值最大（32.2 kg)，大于群体均值1个表型标准差；F09引物的EGF组合对胴体重的效应值为12.53 kg；CY0814引物的BC组合对净肉重的效应值为12.13 kg。 |
| 关键词: 杂种牛 秦川牛 肉用性能 RAPD标记 遗传多样性 屠宰性能 |
| DOI： |
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| 基金项目:国家“863”项目(2001AA243052)，国家自然科学基金(30070551) |
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| RAPD marker of beef performance of the Qinchuan yellow Cattle and its hybrid |
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| Abstract: |
| Genetic varity and butcher performance (live weight/carcass weight/beef wight Qinchuan yellow cattle and its hybrid were analyzed by RAPD marker.The resuts showed that,①13 DNA bands were amplified by the primer of F09 and CY0814,the frequency of polymorphic bands were separately 0.571 4 and 0.833 3 with an average of 0.692.②The relationship between amplified DNA bands and beef performance was showed,the residual quality of LSE between 1 type and 0 type of CY0814D band was 22.17 kg on live weight trait;the ones of LSE between 1 type and 0type of F09 primer were 14.32 kg on carccass weight trait and 11.36 kg on beef weight trait.③The effect of multi-bands combined was studied,the LSE of ABCD combined type of CY0814 primer (32.2 kg)was higher than average about one standard deviation of this population,the LSE of EFG combined type of F09 primer was 12.53 kg on carcass weight and the ones of BC combined type of CY0814 primer were 12.13 kg on beef weight. |
| Key words: Qinchuan yellow cattle beef performance RAPD marker |
