| 摘要: |
| 采用一步法和二步法研究玻璃化冷冻保存的牛生发泡（GV）期和体外成熟（IVM)卵母细胞的发育潜力。结果表明，冷冻基础液PBS和TCM199在玻璃化冷冻中的效果差异不显著（P＞0.05)；冷冻程序和卵母细胞发育阶段对冷冻效果有明显影响，二步法冷冻效果显著优于一步法（P＜0.05)，IVM卵母细胞冷冻效果显著优于GV期（P＜0.05)。 |
| 关键词: 牛 卵母细胞 玻璃化冷冻 |
| DOI： |
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| 基金项目:98留学回国启动基金(4130107)，山东省自然科学基金资助项目(Q99D06) |
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| Vitrificated cryopreservation of bovine oocytes |
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| Abstract: |
| The developmental competences of bovine IVM and GV-stage oocytes cryopreserved by vitrification were studied.The results showed that there was no significant differences between TCM199 and PBS in vitrification as the holding medium (P>0.05),whereas the step of cryopreservation and development stage had significant effects on the development capacity of frozen-thawed bovine oocytes,the development capacity with two-step vitrification was better than that with one step vitrification after thawing.The fertilization rates and the cleavage rates of GV oocytes were significantly lower than that of the IVM oocytes(P<0.05). |
| Key words: bovine oocyte vitrification |
