| 摘要: |
| 结合电镜技术，应用体外全胚胎培养（WEC）方法，观察了黄花棘豆生物碱对大鼠卵黄素（YS）胎盘及胚胎的影响。结果表明，黄花棘豆生物碱质量浓度在50 μg/mL以上时，YS血管分化程度明显减低，对胚胎器官形态学分化的影响有剂量反应关系；质量浓度在200 μg/mL时，YS直径和畸胎数与对照组有显著差异。电镜观察发现，剂量在50 μg/mL以上时，YS上皮细胞微绒毛稀疏变短，胞质中贮液泡减少，线粒体、内质网等内膜系统发生多种细胞病理学改变，细胞间隙增宽，连接复合体破坏。表明YS血管分化及其超微结构对棘豆生物碱反应敏感，其分化程度与对胚胎发育程度的影响一致，可以认为YS结构与功能受损是黄花棘豆生物碱导致胚胎发育毒性所致。 |
| 关键词: 功能 黄花棘豆 生物碱 卵黄囊胎盘 全胚胎培养 |
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| Effects of Oxytropis ochrocephala alkaloid on structure and function of rat yolk sac placenta in vitro |
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| Abstract: |
| The effects of Oxytropis ochrocephalaalkaloid on rat yolk sac (YS) placenta and embryo development in vitro were investigated by using the whole embryo culture(WEC) method and electron microscopy.The results showed that the alkaloid caused remarkable retardation of YS blood vessel differention and embryo development at dose of over 50 μg/mL.Dose-dependent relationship was noticed at concentration of 200 μg/mL,YS diameter was lower than that of control group,and malformation embryon numbers were larger than that of control group.Many pathological changes of the YS ultramicrostructure were observed at dose of over 50 μg/mL.the majour changes include sparse and short and loose microvili of endermal epithelium cells,a reduction in apical microvesicles and subapical storage vacuoles,swilling of the mitochondria and a reduction of disappear in its cristea,a decrease in rough endoplasmic reticulum,abnormation in pattern and structure of nucleus,an increased vacouolation in cells,and extend in the interval of adjacent cells.The resuts indicated that bloot vessel differentiation and ultramicrostructre of YS were sensitive to the alkaloid.Damage of Ys had a high bearing on retardation of embryo development.The injuries of structure and barrier to function of YS placenta was an important mechanism of the alkaloid that caused embryo developmental toxicity. |
| Key words: Oxytropis ochrocephala alkaloid yolk sac placenta whole embryo culture |
