摘要: |
采用改良 B5作为培养基培养乍娜(Zana)单芽茎段,研究了培养基中微量元素、有机成分、激素、光、温条件对其繁殖倍数的影响。提出了改良 B5中微量元素减半、在2000米烛光连续照光下补加自然光照;多次继代培养中插入一次无激素的培养能提高繁殖倍数,获得壮苗。对肌醇促进或抑制茎段生长的作用进行了讨论。 |
关键词: 乍娜试管繁殖,改良B5培养基,单芽茎段,微量元素,有机成分,激素,光,温度 |
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Several Factors Affecting Zana Tube Propagation |
Wang Guiyu Xu Mingshou Biological Deparment Hanzhong Teacgers'''' College
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Abstract: |
The improved B5 was used as culture medium to culture Zana single bud stem section.Also,the effects of trace elements,organic components and hormones in the medium,light and temperature conditions upon the propagation were studied.It was proposed that there be a half reduction of trace elements in the improved B5,and also an addition of natural light in the case of continuous lighting with 2000-meter candle powers.One culture without hormone inserted into several sub-propagations can increase the propagation times and obtaine sturdy seedlings.In addition,the function of inositol stimulat ing or inhibiting the growth of stem section was discussed. |
Key words: Zana grape tube propagation,improved B5 culture medium,single bud stem section,trace element,organic component,hormone,light,temperature |