| 摘要: |
| 【目的】分析刺梨CAD基因家族成员的进化和表达情况，了解其在木质素积累中的作用。【方法】以拟南芥、草莓等植物的肉桂醇脱氢酶（cinnamyl alcohol dehydrogenase，CAD）序列为参考，筛选刺梨（Rosa roxburghii Tratt. ）CAD家族成员，并进行生物信息学分析和CAD家族成员在刺梨果实发育中的表达分析。【结果】刺梨CAD基因家族有9个成员（RrCAD01-RrCAD09），可分为5个亚族，不均匀地分布在2、3、5、6号染色体上，并以2号染色体上分布最为密集（5个）；基因结构分析发现 ，除RrCAD02外 ，其余成员所含外显子数量为5-6个；亚细胞定位和二级结构预测表明，CAD家族蛋白主要定位于细胞质和细胞膜上，以 α-螺旋、β-转角、无规则卷曲为主。基因上游序列分析发现 ，该家族基因普遍存在光诱导元件和脱落酸、水杨酸等激素响应元件。刺梨果实发育过程中的RNA-seq分析发现，RrCAD05的表达变化趋势与木质素含量测定结果及组织化学染色高度一致；在SA和ABA处理下，RrCAD05的表达与CAD活性及木质素含量均呈显著正相关。【结论】刺梨果实发育过程中，RrCAD05基因转录水平与木质素积累动态一致并存在极显著相关性，且不同程度受外源ABA、SA调控，进而影响果实中木质素含量，可能在刺梨果实木质素合成中起着关键作用。 |
| 关键词: 刺梨 CAD基因家族 生物信息学分析 木质素 基因表达 |
| DOI：10. 13207/j. jnwafu. 2026. 10. 015 |
| 分类号: |
| 基金项目:贵州省科技计划项目（黔科合基础 MS［2025］323，黔科合重大［2025］048）；国家自然科学基金项目（32460728）；贵州省科技计划项目（黔科合服企［2024］004-1，黔科合服企［2024］004-2，黔科合平台 KXJZ［2025］036） |
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| Genome⁃wide identification of RrCAD gene family in Rosa roxburghii and its response to exogenous hormones |
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ZHANG Xiaoying1,2, WANG Ye2, LU Min1, MA Wentao1, NAN Hong1, AN Huaming1
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1.College of Agriculture，Guizhou University，Engineering Research Centre of National Forestry and Grassland Administration for Rosa Roxburghii，Guiyang，Guizhou 550025，China;2.Guizhou Botanical Garden，Key Laboratory for Biodiversity Conservation in Karst Mountain Areas of Southwest China，National Forestry and Grassland Administration，Guiyang，Guizhou 550004，China
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| Abstract: |
| 【Objective】The study aims to analyze the evolution and expression patterns of the CAD gene family members in Rosa roxburghii to understand its role in lignin accumulation.【Method】Using the cinnamyl alcohol dehydrogenase（CAD）gene sequences from Arabidopsis thaliana，strawberry and other plants as references，the CAD family members of R.roxburghii were screened. Bioinformatics analysis and expression analyses of these members during fruit development of R.roxburghii were conducted.【Result】The CAD gene family of R.roxburghii comprised nine members（RrCAD01-RrCAD09），which were classified into five subfami?lies and distributed unevenly on chromosomes 2，3，5 and 6，with the highest density on chromosome 2.Gene structure analysis showed that，with the exception of RrCAD02，all members contained 5-6 exons.Subcellular localization and secondary structure prediction showed that the CAD family proteins were mainly located in the cytoplasm and cell membrane，and their structures were dominated by α-helix，β-turn and random coil. The upstream sequence analysis identified ubiquitous cis-acting elements associated with light induction and hormone responses，including those to abscisic acid（ABA）and salicylic acid（SA）.RNA-seq analysis during fruit devel-opment of R.roxburghii showed that the expression pattern of RrCAD05 was highly consistent with the measured lignin content and histochemical staining results.Furthermore，correlation analysis indicated that under exogenous SA and ABA treatments，the expression level of RrCAD05 was significantly positively correlated with both CAD enzyme activity and lignin content.【Conclusion】During the fruit development of R.roxburghii，the transcript level of RrCAD05 was not only highly consistent with but also significantly correlated with the dynamic accumulation of lignin. Furthermore，its expression was regulated by exogenous ABA and SA to varying degrees，affecting the fruit lignin content，which suggests that RrCAD05 played a critical role in lignin synthesis of R.roxburghii fruit. |
| Key words: Rosa roxburghii CAD gene family bioinformatics analysis lignin gene expression |
