| 摘要: |
| 【目的】筛选适宜的宿主植物和培养基质，构建丛枝菌根真菌（AMF）的高效扩繁体系。【方法】以幼套近明球囊霉（Claroideoglomus etunicatum，Ce）和摩西斗管囊霉（Funneliformis mosseae，Fm）两种 AMF 为试验材料，
选用8种宿主植物和3种基质对两种AMF进行扩繁，测定AMF对宿主植物的侵染程度、孢子密度以及地下干质量和根系活力，筛选出最佳的扩繁宿主植物和基质。利用筛选出的宿主植物与培养基质对3个葡萄果园（宁夏志辉源石酒庄（ZH）、西鸽酒庄（XG）、立兰酒庄（LL））土著AMF菌群进行富集，测定土著AMF菌群对宿主植物的侵染程度，并对土著AMF菌群进行了鉴定。【结果】Ce和Fm两种AMF均能侵染定殖于8种植物，其中在宿主玉米（Zea mays）和三叶草（Tifolium repens）的侵染程度较明显，栽植培养60 d后Ce和Fm孢子密度分别可达770，605和745，540 个/g，总定殖率分别为50.0%，44.0%和56.0%，49.0%。利用玉米和三叶草作为宿主植物，将其分别在3种基质中培养，当玉米在沙土混合基质中栽植时，Ce和Fm的扩繁效果最佳，孢子密度可达700和760 个/g，总定殖率可达47.83%和50.17%。利用筛选的宿主植物和培养基质对3个葡萄果园（LL、ZH、和 XG）土著AMF菌群进行富集，其中玉米对LL果园土著AMF菌群的富集程度最高，总定殖率为59.67%。形态学鉴定结果显示，3个果园扩繁的土著AMF菌群包含5属18种，其中球囊霉属（Glomus）为优势菌属。【结论】在本试验条件下，AMF扩繁的最佳宿主植物是玉米，其次是三叶草，最适基质是沙土混合基质，两者组合可高效扩繁果园土著AMF菌群。 |
| 关键词: 丛枝菌根真菌（AMF） 宿主植物 培养基质 孢子密度 土著 AMF 菌群 |
| DOI：10. 13207/j. jnwafu. 2026. 10. 016 |
| 分类号: |
| 基金项目:宁夏重点研发计划项目“贺兰山东麓酿酒葡萄根部病害生物防控专用菌剂的研制与应用”（2023BCF01026） |
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| Effects of different host plants and medium on AMF inoculant propagation efficiency |
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MA Siyuan, LIU Guanlan, YAN Siyuan, YUAN Qi, XIN Ying, GU Peiwen
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School of Agriculture，Ningxia University，Yinchuan，Ningxia 750021，China
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| Abstract: |
| 【Objective】This study aimed to identify suitable host plants and medium to develop an efficient AMF inoculant propagation system.【Method】With two AMF strains?Claroideoglomus etunicatum（Ce）and Funneliformis mosseae（Fm）as test material，eight host plants and three medium were selected for AMF propagation.AMF colonization，spore yield，belowground biomass，and root activity were determined to identify the optimal host and medium for propagation. The identified host plants and medium were then used to enrich AMF communities from three orchard（Ningxia Zhihui Yuanshi Winery（ZY），Xige Winery（XG），Lilan Winery（LL）） soils，and the infection rate，propagation efficiency，and community composition were analyzed.【Result】AMF inoculants Ce and Fm infected and colonized all eight plant species，with the highest infection rates observed in maize（Zea mays）and clover（Tifolium repens）.Ce colonization rate was 50.0% in maize and 44.0% in clover，and Fm colonization rate was 56.0% in maize and 49.0% in clover. After 60 days，Ce spore densities in maize and clover reached 770 and 605 spores/g，and Fm spore densities in maize and clover reached 745 and 540 spores/g. As host plants，maize and clover were cultured in three medium. The best propagation efficiency for Ce and Fm occurred in maize cultured in sandy growing medium，with colonization rates of 47.83% and
50.17%，and spore densities of 700 and 760 spores/g，respectively. Enrichment of native AMF communities from orchards LL，ZH，and XG under optimal conditions showed that the highest colonization rate（59.67%） was observed in LL. Morphological analysis identified 5 genera and 18 species，with Glomus as the dominant genus.【Conclusion】Under the experimental conditions，maize was the most effective host plant for AMF propagation，and the sandy growing medium was the optimal substrate. The combination of maize and sandy growing medium can effectively enrich the native AMF communities from orchard soils. |
| Key words: arbuscula rmycorrhizal fungi（AMF） host plants medium spore density indigenous AMF community |
