| 摘要: |
| 【目的】对向日葵列当（Orobanche cumana）毛蕊花糖苷提取工艺进行优化，并研究其抗氧化活性，为将其作为药材开发利用提供参考。【方法】从3株产木质素降解菌（雷斯青霉（Penicillium raistrickii）、产红青霉（Penicillium rubens）和霉菌（Fungal sp.））和2株产纤维素降解菌（白腐菌（Phanerochaetc chrysosporium）和黄孢原毛平革菌（Phanerochaete chrysosporium））中筛选最优复合菌剂对向日葵列当进行生物破壁发酵处理，通过气相色谱 质谱联用仪（GC-MS）分析复合菌剂降解后向日葵列当中的有效成分；以毛蕊花糖苷提取量为考察指标，以酵解温度、pH值和酵解时间为考察因素，设计单因素试验及响应面优化试验，优化毛蕊花糖苷提取工艺；体外检测毛蕊花糖苷对OH·自由基、DPPH自由基和超氧阴离子的清除能力，分析毛蕊花糖苷的抗氧化活性。【结果】在向日葵列当中共检测出207种化学物质，含量较高的药效成分为苯乙醇苷类物质毛蕊花糖苷，相对含量为2.470%。以雷斯青霉、白腐菌、黄孢原毛平革菌组合作为最优复合菌剂破壁酵解向日葵列当提取毛蕊花糖苷的最佳条件为：酵解温度27 ℃，酵解pH值7，酵解时间3.5 d,在此条件下，向日葵列当中毛蕊花糖苷的提取量为1 396 μg/mL。向日葵列当毛蕊花糖苷对OH·自由基、DPPH自由基和超氧阴离子有一定的清除能力，抗氧化活性明显，且呈剂量依赖性。【结论】向日葵列当中毛蕊花糖苷含量较高，通过复合菌剂发酵破壁处理能够有效提高毛蕊花糖苷的提取效率，且毛蕊花糖苷有明显的抗氧化活性。 |
| 关键词: 向日葵列当 毛蕊花糖苷 生物破壁 提取工艺 抗氧化活性 |
| DOI： |
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| 基金项目:国家自然基金青年科学基金项目（31201470）；国家自然科学基金项目（J1210069）；哈尔滨市应用科技研究与开发项目（2015RAQXJ012） |
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| Extraction optimization and antioxidant activities of acteoside from Orobanche cumana |
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KONG Dexing,XU Yongqing,YANG Yan,et al
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| Abstract: |
| 【Objective】The extraction process of Orobanche cumana acteoside was optimized and its antioxidant activity was studied to provide basis for its development and application as medicinal materials.【Method】Three lignin-producing degradation bacteria (Penicillium raistrickii,Penicillium rubens and Fungal sp.) and two cellulose producing degradation bacteria (Phanerochaetc chrysosporium and Phanero chaete chrysosporium) were used for bio-wall breaking fermentation of O. cumana,and the active components of O. cumana were analyzed by GC-MS.The single factor test and response surface optimization test were designed to optimize the extraction process of acteoside using the extraction amount as evaluation index and fermentation temperature,pH value and fermentation time as factors.The scavenging ability of acteoside against OH· radical,DPPH and superoxide anion was detected in vitro to analyzed its antioxidant activity.【Result】A total of 207 chemical substances were detected in O. cumana,and the most effective component was phenylethanol glycoside with relative content of 2.470%.The optimal extraction conditions were fermentation temperature 27 ℃,pH 7 and fermentation time 3.5 d.Under these conditions,the extraction amount of acteoside from O. cumana was 1 396 μg/mL.O. cumana acteoside had scavenging ability on OH· radical,DPPH and superoxide anion,and the antioxidant activity was in a dose dependent manner.【Conclusion】The content of acteoside in O. cumanawas high,and the extraction efficiency was effectively improved by fermentation and wall breaking treatment with compound bacterial agent.The obtained acteoside had antioxidant activity. |
| Key words: Orobanche cumana acteoside cell wall breaking extraction process antioxidant activity |
