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氨氮胁迫对刺参“鲁海1号”非特异性免疫的影响
赵 斌1, 周红学2, 李成林,等1
1.山东省海洋科学研究院;2.山东省农业农村厅
摘要:
【目的】研究氨氮胁迫对刺非特异性免疫的影响,为探明刺参新品种对环境中氨氮变动的适应性提供依据。【方法】探究0(氨氮质量浓度低于0.05 mg/L的自然海水),2,4,6,8和10 mg/L氨氮胁迫下体质量分别为(30.2±0.2) g/头(A 组)、(59.2±0.8) g/头(B组)刺参“鲁海1号”存活、生长及体腔液中超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、酸性磷酸酶(ACP)和碱性磷酸酶(AKP)活性的变化。【结果】氨氮胁迫对刺参的存活与生长影响显著,当氨氮质量浓度为10 mg/L时,2组刺参均出现排脏、化皮和死亡个体,A组刺参存活率最低,为87.2%。刺参体质量与特定生长率(SGR)随着氨氮质量浓度的升高呈下降趋势,当氨氮质量浓度为4,6,8和10 mg/L时A、B组刺参SGR均显著低于对照组(P<0.05)。随着胁迫时间的延长,不同氨氮质量浓度下刺参SOD、CAT、ACP和AKP活性总体呈先升高后降低趋势。氨氮胁迫第5天,氨氮质量浓度为2,4 mg/L时部分试验组SOD、CAT活性达到峰值。氨氮胁迫第15天,氨氮质量浓度为8,10 mg/L的试验组SOD、CAT、ACP和AKP酶活性均低于对照组,且多数出现显著差异(P<0.05)。【结论】刺参养殖过程中的水环境氨氮质量浓度应控制在6 mg/L以下,以降低其对刺参造成的不良影响。
关键词:  刺参“鲁海1号”  氨氮胁迫  存活率  非特异性免疫酶
DOI:
分类号:
基金项目:国家重点研发计划项目“蓝色粮仓科技创新”重点专项(2018YFD0901602);山东省泰山产业领军人才工程项目(LJNY201613);山东省农业重大应用技术创新项目
Effects of ammonia nitrogen stress on activities of non-specific immune enzymes of sea cucumber Luhai No.1
ZHAO Bin,ZHOU Hongxue,LI Chenglin,et al
Abstract:
【Objective】This study explored the adaptability of a new variety of sea cucumber (Apostichopus japonicus) to changes of ammonia nitrogen in environment.【Method】The weights of sea cucumbers were (30.2±0.2) g/ind and (59.2±0.8) g/ind and the concentrations of ammonia nitrogen were 0,2,4,6,8 and 10 mg/L.The survival and growth of sea cucumbers and changes in activities of four non-specific immune enzymes (SOD,CAT,ACP and AKP) in coelomic fluid were studied.【Result】Ammonia nitrogen stress had significant effects on survival rate and growth of sea cucumber Luhai No.1.When the concentration of ammonia nitrogen was 10 mg/L,both groups showed excreted internal organs,melted skin and death.The sea cucumbers in group A with 10 mg/L ammonia nitrogen had the lowest survival rate of 87.2%.Body weight and specific growth rate (SGR) of sea cucumbers decreased with the increase of ammonia nitrogen concentration.The SGR of 4,6,8 and 10 mg/L ammonia nitrogen groups was significantly lower than that of the control group (P<0.05).With the increase of exposure time,activities of SOD,CAT,ACP and AKP of sea cucumbers at different concentrations of ammonia nitrogen increased first and then decreased.On the 5th day of ammonia nitrogen stress,activities of SOD and CAT in some experimental groups with 2 and 4 mg/L ammonia nitrogen reached peaks.On the 15th day of ammonia nitrogen stress,activities of SOD,CAT,ACP and AKP in groups with 8 and 10 mg/L ammonia nitrogen were lower than those in the control group,and most of them showed significant differences (P<0.05).【Conclusion】The concentration of ammonia nitrogen in aquaculture environment should be controlled below 6 mg/L to reduce adverse effects on sea cucumber Luhai No.1.
Key words:  sea cucumber Luhai No.1  ammonia nitrogen stress  survival rate  non-specific immune enzymes