| 摘要: |
| 【目的】研究氯甲酚纳米乳消毒剂（CND）对真菌的杀菌效果及其作用机理。【方法】以白色念珠菌为指示菌，分别利用营养肉汤稀释法、悬液定量杀菌试验法、物体表面消毒模拟现场试验法、扫描和透射电子显微镜观察法，研究CND的体外抗菌活性、杀菌效果、模拟现场消毒效果及对真菌超微结构的影响。【结果】CND对白色念珠菌的MIC和MBC均为3 mg/mL。对白色念珠菌的杀菌效果随着其体积分数的增大和作用时间的延长而增强，体积分数0.5％ CND作用15 min、0.6% CND作用10 min和0.8％ CND作用5 min时，消毒合格；0.8％和1.0% CND分别作用10 和5 min时，杀菌率均为100%。体积分数0.2%和0.5% CND的杀菌率极显著高于相同体积分数的氯甲酚溶液（P＜0.01），二者体积分数为1.0%时差异不显著（P＞0.05）。经体积分数0.8% CND作用10 min 后，扫描电镜下白色念珠菌表面粗糙不平，皱褶明显，部分菌体表面出现裂缝；透射电镜下菌体形状发生改变，细胞壁变薄，且部分细胞壁局部有断裂或破损，部分真菌的细胞膜和细胞核边界不清，结构模糊，多数真菌的细胞质内部构造不清，不同真菌之间的胞浆内电子密度不同。【结论】CND对真菌的杀菌作用强，并能使白色念珠菌的超微结构发生改变。 |
| 关键词: 氯甲酚纳米乳 消毒剂 白色念珠菌 杀菌作用 超微结构 |
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| 基金项目:河南科技学院“标志性创新工程”计划经费项目（2015ZD09）；河南省高等学校大学生创新训练计划项目（S20191-0467006）；河南科技学院大学生创新训练计划项目（2019CX084） |
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| Fungicidal effect of chlorcresol nanoemulsion disinfectant |
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YIN Mei,ZHANG Daoliang,SUN Yanju,et al
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| Abstract: |
| 【Objective】The aim of this paper was to study the fungicidal effect and mechanism of chlorocresol nanoemulsion disinfectant (CND).【Method】Taking Candida albicans as the indicator fungus, the nutritional broth dilution method,suspension quantitative fungicidal test,object surface disinfection simulation field test,observational method with scanning and transmission electron microscope were used to investigate the in vitro antifungal activity, fungicidal action,field simulation effect of CND and fungal ultrastructural changes caused by CND.【Result】MIC and MBC of CND against Candida albicans were both 3 mg/mL.The fungicidal action increased with the increase of CND concentration and the extension of disinfection time.The disinfection was qualified when the volume fractions of 0.5%,0.6% and 0.8% CND were acted for 15,10 and 5 minutes, respectively.When 0.8% and 1.0% CND were disinfected for 10 and 5 minutes,the fungicidal rate was 100%.The fungicidal rates of volume fractions of 0.2% and 0.5% CND were significantly higher than that of clocresol solution (P<0.01),while the difference was not significant at the volume fraction of 1.0% (P>0.05).After Candida albicans was treated by volume fraction of 0.8% CND for 10 min,the surface was rough with obvious wrinkles,and cracks appeared on the surface under scanning electron microscope.Under transmission electron microscope,the shape changed with thinner and partially broken or locally damaged cell walls.The boundary of cell membrane and nucleus were unclear and the structure was ambiguous.The internal cytoplasm construction of most fungi was not clear. Different fungi had different electron densities in cytoplasm.【Conclusion】CND had strong fungicidal effect on fungi and could change ultrastructure of Candida albicans. |
| Key words: chlorocresol nanoemulsion disinfectant Candida albicans fungicidal action ultrastructure |
