| 摘要: |
| 【目的】对分离自林麝化脓肺脏的1株疑似铜绿假单胞菌（PA）进行鉴定，并分析其致病和耐药机制，为林麝PA感染化脓性疾病的防控奠定基础。【方法】将病原菌分离纯化后，依次进行生化试验、16S rRNA序列分析、药敏试验和小鼠致病性试验，并通过全基因组测序，对分离菌株进行群体进化和物种分型分析以及基因功能注释。【结果】分离自林麝化脓肺脏的1株疑似铜绿假单胞菌经鉴定与PA相符，命名为FMDP001。药敏试验显示其对阿莫西林、头孢曲松、氨曲南、多粘菌素B和林可霉素耐药；对小鼠半数致死量为9.4×105 CFU/mL。全基因组序列分析显示该菌基因组大小为6 955 100 bp，序列类型为ST1249，与B136 33株同源性最高，且两菌株基因组平均核苷酸一致性（ANI）值达98.93%；全基因组中共有357个序列编码FMDP001与致病性相关的基因，根据功能分为黏附、铁摄取、胞外毒性蛋白和调控系统；84个序列编码耐药基因，其中多药耐药外排泵为主要成分。【结论】从林麝化脓肺脏中分离到1株致病性较强的PA，并获得ST1249型林麝源PA的全基因组序列，序列显示该菌携带大量药物外排泵及生物膜形成相关基因，决定其具有多重耐药特性，哌拉西林等可作为该类型PA感染的临床用药。 |
| 关键词: 林麝 铜绿假单胞菌 全基因组序列分析 致病性 耐药性 |
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| 基金项目:四川省科技厅2020年四川省科研院所科技成果转化资金项目；四川省科技厅应用基础研究项目（2018JY0644） |
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| Isolation,identification and whole genome sequence analysis of Pseudomonas aeruginosa (ST1249) from lung of forest musk deer |
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WU Xi,YANG Wei,ZHAO Wei,et al
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| Abstract: |
| 【Objective】A strain of Pseudomonas aeruginosa (PA) isolated from the pyogenic lung of forest musk deer(FMD) was identified and its pathogenesis and drug resistance mechanism were analyzed to provide basis for the prevention and control of suppurative disease caused by PA infection in FMD.【Method】Biochemical tests,16S rRNA analysis,drug susceptibility tests,and pathogenicity tests in mice were conducted after isolation and purification of pathogens.Through the genome-wide sequencing,the population evolution and species typing were analyzed and gene function annotations were carried out.【Result】A strain from the pyogenic lung of FMD was identified to be consistent with PA,and was named FMDP001.Drug susceptibility tests showed that it had resistance to amoxicillin,ceftriaxone,aztreonam,polymyxin B and lincomycin with 50% lethal dose (LD50) of 9.4×105 CFU/mL.Genome sequence analysis showed that the genome size was 6 955 100 bp and the sequence type was ST1249,which had the highest homology with B136-33 strains with average nucleotide identity (ANI) of 98.93%.There were 357 sequences encoding FMDP001 related to pathogenicity in the whole genome,and they were divided into groups of adherence,iron uptake,extracellular toxic protein and regulation system according to functions.There were 84 sequences encoding drug resistance genes and efflux pump was the main component.【Conclusion】 A strong pathogenic PA (ST1249) was isolated from the purulent lung of FMD and its whole genome sequence was obtained.The bacteria carried many genes related to drug efflux pump and biofilm formation,which determined its multi drug resistance.Piperacillin can be used as a clinical drug for such PA infection. |
| Key words: forest musk deer Pseudomonas aeruginosa genome sequence analysis pathogenicity drug resistance |
