| 摘要: |
| 【目的】建立紫萁（Osmunda japonica Thunb.）孢子叶组织培养快繁体系，筛选其适宜的组织培养条件。【方法】以紫萁孢子叶穗为外植体，研究乙醇和HgCl2处理不同时间对外植体的消毒效果；在加入0.3 mg/L NAA的1/4MS培养基中，再分别加入0.5 mg/L的2,4-D、IBA和KT，比较各处理对原叶体的诱导效果；在1/2MS基础培养基上，采用L9(34)正交试验设计，探讨不同质量浓度KT、GA-3、NAA和6-BA组合对原叶体增殖的影响；同时，研究光合酵素稀释300，500和700倍对紫萁孢子体诱导的影响，以及1/2MS 培养基中加入0.1，0.5，1.0 mg/L IBA或NAA对紫萁组培苗生根的影响。【结果】使用1 g/L HgCl2对紫萁孢子叶穗消毒8 min的效果较好，萌发率为61.11%；紫萁原叶体最适宜的诱导培养基为1/4MS+KT 0.5 mg/L+NAA 0.3 mg/L，诱导率为88.55%；紫萁原叶体最适宜的增殖培养基为1/2MS+KT 5 mg/L+GA3 3 mg/L+NAA 1 mg/L，增殖系数为9.6；稀释500倍的光合酵素对紫萁孢子体苗的转化及生长有促进作用，孢子体转化率为57.23%，孢子体高度为2.33 cm；1/2MS 培养基中加入0.5 mg/L IBA，紫萁组培苗生根率与长势俱佳，生根率可达93.33%。【结论】初步建立了紫萁孢子适宜的组织培养体系，在相应条件下组培苗长势良好。 |
| 关键词: 紫萁 组织培养 孢子繁殖 激素诱导 |
| DOI： |
| 分类号: |
| 基金项目:吉林省科技厅科技支撑计划项目“特殊蔬菜（含山野菜类）品种选育、栽培技术研究”（20180201079NY） |
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| Tissue culture of osmunda spore leaves |
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SUN Xiaodan,LI Chunpeng,DONG Ran,et al
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| Abstract: |
| 【Objective】This study aimed to establish a rapid propagation system for tissue culture of osmunda spore leaves and screen suitable conditions.【Method】The spores of osmunda were used as explants to study the disinfection effect of ethanol and HgCl2 on explants with different treatment times.In the 1/4 MS culture medium with 0.3 mg/L NAA,2,4 d,IBA and KT at 0.5 mg/L were added,respectively,and the induction effects on protoplasts were compared.On 1/2MS basal medium,the L9(34) orthogonal design was used to investigate the effects of KT,GA-3,NAA and 6-BA at different concentrations on proliferation of protoplasts.The effects of 300,500 and 700 times dilution of photosynthetic enzymes on induction of osmunda sporophyte and the effect of 0.1,0.5,1.0 mg/L IBA or NAA on rooting of osmunda tissue culture seedlings in 1/2MS medium were also investigated.【Result】Sterilization with 1 g/L HgCl2 for 8 min was better with germination rate of 61.11%.The most suitable induction medium for osmunda protoplasts was 1/4MS+KT 0.5 mg/L+NAA 0.3 mg/L with induction rate of 88.55%.The most suitable proliferation medium for osmunda protoplasts was 1/2MS+KT 5 mg/L+GA3 3 mg/L+NAA 1 mg/L with proliferation coefficient of 9.6.The photosynthetic enzyme diluted by 500 times promoted the transformation and growth of osmunda spore seedlings with sporophytic transformation rate of 57.23% and spore body height of 2.33 cm. Adding 0.5 mg/L IBA to 1/2MS medium improved rooting rate and growth of osmunda tissue culture seedlings and the rooting rate reached 93.33%.【Conclusion】A suitable tissue culture system for osmunda spores was initially established,and the tissue culture seedlings grew well under corresponding conditions. |
| Key words: osmunda tissue culture spore reproduction hormone induced |
