| 摘要: |
| 【目的】了解新疆乌鲁木齐市宠物犬源沙门氏菌和葡萄球菌的耐药性及耐药基因携带情况。【方法】从77份宠物犬直肠粪便样品中分离沙门氏菌和葡萄球菌，采用琼脂稀释法对分离的菌株进行药敏试验，通过PCR方法对沙门氏菌6类23种耐药基因和葡萄球菌5类9种耐药基因进行检测。【结果】从77份宠物犬粪便样品中分离到沙门氏菌36株，葡萄球菌75株。除头孢噻呋、安普霉素、阿米卡星、硫酸庆大霉素和多黏菌素外，宠物犬源沙门氏菌对其他被捡抗菌药物的耐药率高达97.0%以上，多重耐药以8耐为主，tetB、blaTEM、blaOXA、qnrS、oqxA、oqxB、aac(6′)Ib-cr、ant(3″)Ia和floR基因检出率在97.0%以上。宠物犬源葡萄球菌对左氧氟沙星(93.5%)、苯唑西林(90.7%)和氟苯尼考(82.9%)耐药率在80.0%以上，多重耐药集中在4耐、6耐和9耐，主要检出的基因为fexA(40.0%，30/75)和femA(18.7%，14/75)，其次为tetM(16.0%，12/75)、ermB(14.7%，11/75)和mecA(1.3%，1/75)。【结论】宠物犬源沙门氏菌和葡萄球菌耐药情况严重，耐药基因携带率高，使宠物犬的细菌性疾病治疗难度加大，应加强耐药菌的监测，降低宠物犬源耐药菌转移给人的潜在风险。 |
| 关键词: 宠物犬 沙门氏菌 葡萄球菌 耐药性 耐药基因检测 |
| DOI： |
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| 基金项目:国家自然科学基金 新疆联合基金项目（U1503185） |
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| Detection of drug resistance and resistant genes of Salmonella and Staphylococcus from pet dog |
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XUAN Huiyong,LIN Yajun,GAO Chao,et al
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| Abstract: |
| 【Objective】This study investigated the resistance and resistant genes of Salmonella and Staphylococcus isolates from feces of pet dog against antibacterial drugs in Urumqi,Xinjiang.【Method】Salmonella and Staphylococci were isolated from 77 pet dog rectal feces samples.Agar dilution method was used to test the susceptibility of the isolates.The PCR method was used to detect 23 resistant genes of 6 kinds of Salmonella and 9 resistant genes of 5 kinds of Staphylococcus.【Result】A total of 36 strains of Salmonella and 75 strains of Staphylococcus were isolated.Except ceftiofur,apramycin,amikacin,gentamicin and polymyxin,the resistant rates of Salmonella isolates against other detected antimicrobial agents were above 97.0%.The multidrug resistance strains mainly focused on 8 kinds of drug resistance, and the detection rates of tetB,blaTEM,blaOXA,qnrS,oqxA,oqxB,aac(6′)-Ib-cr,ant(3″)-Ia and floR genes were larger than 97.0%.The resistant rates of Staphylococcus against levofloxacin (93.5%),oxacillin (90.7%) and florfenicol (82.9%) were higher than 80.0%.The multidrug resistance results of Staphylococcus were concentrated on 4 types,6 types and 9 types.The main detected genes were fexA (40.0%,30/75) and femA (18.7%,14/75),followed by tetM(16.0%,12/75),ermB(14.7%,11/75) and mecA (1.3%,1/75).【Conclusion】The drug resistance of Salmonella and Staphylococcus was severe,and the resistance gene-carrying rates were high,resulting in difficult treatment of bacterial diseases in pet dogs.The monitoring of drug-resistant bacteria should be strengthened to reduce the potential risk of transfer of drug resistant bacteria from pet dogs. |
| Key words: pet dog Salmonella Staphylococcus resistance detection of resistance genes |
