摘要: |
【目的】研究绵羊STAT5a基因第10内含子和第12外显子的遗传多态性和群体遗传特征。【方法】以2个多羔绵羊品种湖羊(140只)和多浪羊(144只)及单羔羊藏绵羊(217只)为研究对象,应用PCR-SSCP方法分析其STAT5a基因第10内含子和第12外显子的遗传多态性和群体遗传特征。【结果】绵羊STAT5a基因第10内含子发现13 210 A>G突变,检测到3种基因型(AA、AG和GG)和2个等位基因(A和G);第12外显子上发现14 827 A>G突变,该突变属于同义突变,检测到3种基因型(MM、MN和NN)和2个等位基因(M和N)。在绵羊STAT5a基因第10内含子和第12外显子中,杂合子AG和MN分别为优势基因型,基因型频率在多羔绵羊和单羔绵羊品种间差异极显著(P<0.01)。3个绵羊品种的多态信息含量均处于中度多态,藏绵羊的卡方值处于Hardy Weinberg平衡状态,而湖羊和多浪羊偏离了Hardy-Weinberg平衡状态。【结论】绵羊STAT5a基因具有多态性,且基因型频率在不同产羔数品种间差异极显著(P<0.01)。 |
关键词: 绵羊 STAT5a基因 遗传多态性 PCR-SSCP |
DOI: |
分类号: |
基金项目:甘肃省基础研究创新群体项目(17JR5RA137);甘肃农业大学“伏羲青年英才”培育计划项目(Gaufx-02Y02);甘肃省高校基本科研业务项目;甘肃农业大学自列课题(GSAU-ZL-2015-033) |
|
Genetic characteristics of intron 10 and exon 12 of STAT5a gene in sheep |
HAO Zhiyun,WANG Jiqing,ZHAO Mengli,et al
|
Abstract: |
【Objective】This study investigated the genetic polymorphism and population genetic characteristics of intron 10 and exon 12 of STAT5a in sheep.【Method】A total of 140 Hu sheep and 144 Duolang sheep of two polygamous sheep breeds as well as 217 Tibetan sheep of single lambs were selected and the PCR SSCP was used to analyze the genetic polymorphism and population genetic characteristics of intron 10 and 12 exon of the STAT5a gene.【Results】The 13 210 A>G mutation was found in the intron 10 of STAT5a gene including three genotypes (AA,AG and GG) and two alleles (A and G).The 14 827 A>G synonymous mutation including three genotypes(MM,MN and NN) and two alleles (M and N) were detected in the exon 12.Heterozygous AG and MN were dominant genotypes in intron 10 and exon 12.The frequencies of genotypes were significantly different among multiple lambs and single lambs (P<0.01).The polymorphic information of the three sheep breeds was all moderately polymorphic.The Chi square value of Tibetan sheep was in Hardy-Weinberg equilibrium,while that of Hu sheep and Duolang sheep deviated from Hardy Weinberg equilibrium. 【Conclusion】STAT5a was polymorphic and the genotype frequencies of lambs were significantly different among different litter sizes (P<0.01). |
Key words: sheep STAT5a gene genetic polymorphism PCR-SSCP |