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内蒙古绒山羊皮肤KRTAPs表达谱与日照时长的相关性
赵 濛,奈日乐,谢遇春,等
作者单位
赵 濛 内蒙古农业大学 动物科学学院 
奈日乐 内蒙古农业大学 动物科学学院 
谢遇春,等 内蒙古农业大学 动物科学学院 
摘要:
【目的】研究绒山羊皮肤部分角蛋白关联蛋白(keratin associated protein,KRTAPs)基因的表达谱及其与全年日照变化规律间的相关性。【方法】以3只周岁阿尔巴斯型内蒙古绒山羊体侧皮肤为样本,进行高通量转录组测序(RNA-Seq),对获得的部分KRTAPs基因一年内不同月份间的转录本进行表达丰度分析,并结合当地全年日照变化规律与RT-PCR试验方法,验证KRTAP24-1、KRTAP3-1、KRTAP8-1在关键节点月份的表达谱,确定全年皮肤KRTAPs表达丰度变化与日照变化规律间的相关性。【结果】Illumina solexa高通量转录组测序表明,内蒙古绒山羊全年皮肤部分KRTAPs基因的表达具有规律性差异。RT-PCR试验验证表明,部分基因表达谱与测序表达丰度具有一致性,并且基因差异变化规律与全年日照时长的变化具有相关性。【结论】全年日照时长由长变短(夏-冬)时皮肤KRTAPs表达丰度上调,日照时长由短变长(冬-夏)时皮肤KRTAPs表达丰度下调。
关键词:  绒山羊  角蛋白关联蛋白  转录水平  绒毛周期
DOI:
分类号:
基金项目:国家自然科学基金项目(31360537,31272421);国家“863”计划项目(2013AA102506)
Relationship between expression pattern of keratin-associated proteins in Arbas cashmere goat and sunshine duration
ZHAO Meng,NAI Rile,XIE Yuchun,et al
Abstract:
【Objective】The expression patterns of skin keratin-associated proteins (KRTAPs) in Arbas cashmere goat were investigated and the relationship with sunshine duration was analyzed. 【Method】The skin of three one year old Arbas cashmere goat was used for high-throughput sequencing and the obtained information was used for abundance analysis.Then the yearly solar radiation change and RT-PCR technique were used to verify the expressions of KRTAP24-1,KRTAP3-1,and KRTAP8-1.The relationship between expression abundance and solar radiation duration was also analyzed. 【Result】The KRTAPs expression pattern had certain regularity based on illumine solexa sequencing.RT-PCR showed that some expression profiles were identical to expression abundance and the variations of gene expression were related with solar radiation duration. 【Conclusion】When the sunshine duration lengthens (summer to winter),the KRTAPs expression increases while it decreases when sunshine duration shortens (winter to summer). 
Key words:  cashmere goat  keratin associated protein  transcriptional expression level  hair growth cycle