| 摘要: |
| 【目的】了解八仙花HmVALT基因表达与耐铝能力的关系，揭示八仙花耐铝机理。【方法】以耐铝的八仙花品种H.macrophylla cv ‘Coerulea’为材料，扩增获得HmVALT基因片段，利用半定量RT-PCR方法，分析在0，50，100，200，400，600 μmol/L AlCl3胁迫下，八仙花根和叶中HmVALT基因的表达情况。【结果】各处理HmVALT基因在八仙花根和叶部均有表达，且根部的表达量高于叶部；铝胁迫浓度为400 μmol/L时八仙花根和叶中HmVALT基因表达量最高，随后降低。【结论】HmVALT基因的表达与八仙花耐铝性密切相关。 |
| 关键词: 八仙花 耐铝能力 HmVALT基因 半定量RT-PCR |
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| 基金项目:国家自然科学基金项目(31201656)；湖南省教育厅青年基金项目(14B093)；湖南省林业厅科技计划项目(XLK201338) |
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| Difference in HmVALT gene expression of Hydrangea macrophylla under different aluminum concentrations |
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CHEN Haixia,FAN Shi,HU Chunmei
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| Abstract: |
| 【Objective】This experiment investigated the relationship between expression of HmVALT gene and aluminum resistance of Hydrangea macrophylla to provide basis for study on aluminum tolerant mechanism of H.macrophylla.【Method】The HmVALT gene segment was obtained from H.macrophylla cv‘Coerulea’ via RT-PCR.Semi-quantitative RT-PCR was used to analyze the expression variation of HmVALT gene under aluminum stress with different concentrations of 0, 50,100,200,400,and 600 μmol/L. 【Result】The expression of HmVALT gene was found in all treatments,and the expression level in root was higher than that in leaf.The highest expression was obtained with AlCl3 concentration of 400 μmol/L,and then it decreased.【Conclusion】The expression of HmVALT gene was closely related to aluminum tolerance of H.macrophylla. |
| Key words: Hydrangea macrophylla aluminum resistant HmVALT gene semi-quantitative RT-PCR |
