| 摘要: |
| 【目的】研究盐酸水解法提取林蛙卵黑色素的最佳工艺，并分析林蛙卵黑色素的抗氧化活性。【方法】以林蛙卵为原料，通过单因素试验和正交试验，分析盐酸浓度、原料与盐酸料（g）液(mL)比、水解温度、水解时间对黑色素提取率的影响，确定林蛙卵黑色素盐酸水解法的最佳提取工艺；应用ＤＰＰＨ法、邻二氮菲法及邻苯三酚自氧化检测法，测定林蛙卵黑色素对ＤＰＰＨ、羟基自由基和O2－·的清除能力。【结果】 酸水解法提取林蛙卵黑色素的最佳条件为：盐酸浓度6 mol/L，料液比1∶30，水解温度70 ℃，水解时间2 h，在此条件下，林蛙黑色素提取率达7.02%。黑色素对DPPH自由基具有显著的清除作用，清除50% DPPH自由基对应的黑色素质量浓度为0.6 mg/mL；1.0 mg/mL黑色素对羟基自由基的清除率达到65%；黑色素对O2－·的清除率不佳，最高达28.3%。【结论】盐酸水解法对林蛙卵黑色素的提取效果较好，林蛙卵黑色素具有明显的抗氧化作用。 |
| 关键词: 林蛙卵 黑色素 工艺条件 抗氧化活性 |
| DOI： |
| 分类号: |
| 基金项目:吉林省自然科学基金项目(201115189)；吉林农业大学博士启动基金项目（2012225） |
|
| Extraction and antioxidant activities of melanin from brown frog eggs |
|
SU Yuchun,CHEN Guang,GUO Huinan,et al
|
| Abstract: |
| 【Objective】This study focused on the optimum extraction process of melanin from brown frog eggs by acid hydrolysis and its antioxidant activities.【Method】Using brown frog eggs as material,the effects of hydrochloric acid concentration,material to liquid ratio,hydrolysis temperature and hydrolysis duration were studied through single factor experiments and orthogonal test and the optimum hydrolysis process was determined.The scavenging activities on DPPH,hydroxyl free radical and O2－· of extracted melanin were measured by DPPH method,phenanthroline-Fe (Ⅱ) oxidation assay and pyrogallol oxidation method.【Result】The optimum hydrolysis parameters for melanin extraction from brown frog eggs were:hydrochloric acid concentration 6 mol/L,material (g) to liquid (mL) ratio 1∶30,hydrolysis temperature 70 ℃,and hydrolysis duration 2 h.Melanin extraction yield of up to 7.02% was obtained by the method.Melanin had significant clearance ability on DPPH radicals and its concentration for half clearance rate was 0.6 mg/mL.The clearance ability on hydroxyl radical and O2－· at concentration 1.0 mg/mL were 65% and 28.3%,respectively.【Conclusion】Hydrochloric acid hydrolysis was an efficient way to extract melanin from brown frog eggs,and the extracted melanin had significant antioxidant activities. |
| Key words: brown frog eggs melanin hydrolysis parameter anti oxidation activities |
