| 摘要: |
| 【目的】筛选对亚硝酸盐具有清除作用的药用植物，并研究其对亚硝酸根（NO2－） 的清除率与醇提物总酚含量的相关性，为开发临江地区药用植物资源提供理论依据。【方法】依据NO2－在弱酸性条件下与对氨基苯磺酸重氮化，再与盐酸萘乙二胺偶合生成偶联化合物，用WD-2102A 型自动酶标仪测定药用植物醇提物吸光度，计算NO2－清除率，并对清除率较高的样品进一步测定，并计算其IC50；应用简单的线性回归分析药用植物醇提物对NO2－的清除率与其总酚含量之间的相关性。【结果】38 种药用植物醇提物均对NO2－具有一定的清除作用，但不同药用植物的清除作用差异很大；药用植物醇提物对NO2－的清除率(y)与其总酚含量(x)呈正向相关，相关方程为y=0.915 5x+17.644，相关系数R2=0.323；筛选出对NO2－清除率大于55%的9种药用植物（含同种药用植物不同选取部位）进一步进行测定，结果表明药用植物醇提物对NO2－的清除率随其醇提物质量浓度的增加而增强，其清除作用大小顺序为小花地笋>茜草>野生葡萄（籽）>草本水杨梅>Vc（抗坏血酸）>茶条槭（枝）>仙鹤草>月见草>茶条槭（叶）>茶条槭（果）>宽叶山蒿>意大利苍耳（果）。【结论】临江地区38种药用植物中野生葡萄（籽）和茶条槭最有希望被开发；药用植物中的多酚类成分为其NO2－清除作用的主要活性物质。 |
| 关键词: 药用植物 醇提物 亚硝酸根 清除率 总酚含量 |
| DOI： |
| 分类号: |
| 基金项目:国家公益性行业科研专项(201303111)；吉林省科技发展计划项目(20140204013YY，20150307012YY) |
|
| Nitrite scavenging effect of alcohol extracts of thirty-eight medicinal plants |
|
ZHAO Yan,LENG Yantao,YU Ting,et al
|
| Abstract: |
| 【Objective】This study screened medicinal plants with nitrite scavenging effect and investigated the correlation between NO2－ scavenging rate and total phenolic content of extracts to provide basis for exploitation of medicinal plant resources in Linjiang.【Method】According to sulfanilic acid diazotization of nitrite under acidic condition,coupling compound was formed through reaction with hydrochloric acid naphthyl ethylenediamine.WD-2102A automatic microplate reader was used to determine the absorbance of solution,nitrite clearance was calculated,and samples with high clearance rate were further determined to calculate IC50.Simple linear regression analysis was used to analyze the correlation between NO2－ scavenging rate and contents of total phenols in the extracts.【Result】The ethanol extracts from 38 medicinal plants had different NO2－ scavenging effects.The scavenging rates were positively related to the contents of total phenols following equation Y=0.915 5x+17.644 with correlation coefficient R2 of 0.323.The 9 species with NO2－scavenging rate greater than 55% (including different parts of same plant) were further measured.The NO2－ removal rate increased with the increase of concentrations of alcohol extracts.The NO2－ scavenging abilities of plants were in the decreasing order of Lycopus parviflorus Maxim.>Rubia cordifolia Linn.>Vitis vinifera (seed)>Geum aleppicum Jacq.>Vc>Acer ginnala Maxim.(branch)>Agrimonia pilosa Ledeb.>Oenothera erythrosepala Borb.>Acer ginnala Maxim.(leaf)>Acer ginnala Maxim.(fruit)>Artemisia stolonifera (Maxim.) Komar>Xanthium italicum Moretti(fruit).【Conclusion】Vitis vinifera (seed) and Acer ginnala Maxim.had the most promising potential for development of NO2－ scavenger among the 38 species in Linjiang and polyphenolic constituents were the main active substance for NO2－ scavenging. |
| Key words: medicinal plant alcohol extract NO2－ clearance rate total phenolic content |
