| 摘要: |
| 【目的】考察不同维生素E(VE)含量饲料对胭脂鱼幼鱼生长性能、肝脏抗氧化指标及肝脏VE蓄积量的影响，以确定胭脂鱼幼鱼对饲料中适宜VE的需要量。【方法】 以酪蛋白、明胶、白鱼粉、糊精、大豆油和玉米油等为原料，配制成7组半纯化试验饲料，饲料中VE含量分别为0.51(对照组)，15.23，31.86，62.30，120.32，240.42和483.71 mg/kg，投喂初始体质量为(5.52±0.09) g/尾的胭脂鱼(Myxocyprinus asiatius)幼鱼8周，测定并分析胭脂鱼幼鱼增重率、特定生长率、体成分、肝脏抗氧化指标以及肝脏VE蓄积量。【结果】胭脂鱼幼鱼增重率及特定生长率均随饲料VE含量的增加而呈先上升后下降的趋势，62.30 mg/kg VE组试验鱼显著高于其他各组(P＜0.05)；存活率随饲料VE含量的增加而呈现出先上升后下降的趋势，对照组、15.23 mg/kg VE组的存活率显著低于62.30～240.42 mg/kg VE组(P＜0.05)，而其余各组之间的存活率无显著差异(P＞0.05)；饲料系数（FCR）随饲料VE含量的增加而呈现先下降后上升的趋势；不同VE含量对胭脂鱼幼鱼肝体比、肥满度、脏体比均无显著影响。胭脂鱼幼鱼肌肉粗脂肪含量随饲料VE含量的增加呈先下降后上升的趋势，在饲料VE含量为62.30 mg/kg时最低，肌肉水分、粗蛋白含量及全鱼水分、粗蛋白、粗脂肪和灰分在各处理间无显著差异；肝脏总抗氧化力(T-AOC)和超氧化物歧化酶(SOD)活性随着饲料中VE含量的增加呈先上升后下降的趋势，均在饲料VE含量为31.86 mg/kg时最高；肝脏丙二醛(MDA)含量随着饲料中VE含量的升高显著降低(P＜0.05)。肝脏VE蓄积量随着VE含量的增加呈线性上升。【结论】以特定生长率为评价指标，经折线回归分析，胭脂鱼幼鱼获得最大生长性能时，对饲料VE需要量为50.60 mg/kg。 |
| 关键词: 胭脂鱼幼鱼 维生素E 饲料试验 |
| DOI： |
| 分类号: |
| 基金项目:公益性行业（农业）科研专项（201203086-06） |
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| Dietary vitamin E requirement of juvenile Chinese sucker (Myxocyprinus asiatius) |
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ZHANG Zhiqiang,JIANG Ming,WEN Hua,et al
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| Abstract: |
| 【Objective】The experiment determined the dietary vitamin E requirement of juvenile Chinese sucker by investigating the effects of vitamin E on growth performance,liver antioxidant index and vitamin E accumulation in liver.【Methods】Juvenile Chinese sucker with initial body weight of (5.52±0.09) g were fed with semi-purified diets with casein,gelatin,fish meal,dextrin,corn oil and soy oil as ingredients and containing 7 vitamin E levels (0.51 (the control),15.23,31.86,62.30,120.32,240.42 and 483.71 mg/kg diet) for 8 weeks.Weight gain rate (WGR),specific growth rate (SGR),body and muscle composition,liver antioxidant index of juvenile and vitamin E concentration in liver of juvenile Chinese sucker were then detected and analyzed.【Results】WGR and SGR increased at first and then decreased with the increase of dietary vitamin E level (P＜0.05),and both reached the maximum values at 62.30 mg/kg diet.Survival rate (SR) increased at first and then decreased with the increase of dietary vitamin E level.The SR of the control group and 15.23 mg/kg diet were much lower than that of 62.30－483.71 mg/kg diets,and there were no differences between 62.30－240.42 mg/kg diets.Feed conversion rate (FCR) decreased at first and then increased with the increase of dietary vitamin E level.Condition factor (CF),hepatosomatic (HSI) and viscerosomatic index (VSI) were not affected by the supplemental level of dietary vitamin E.The crude lipid in muscle decreased at first and then increased with the increase of dietary vitamin E level (P＜0.05),and it reached the minimum at 62.30 mg/kg diet.The moisture and crude protein in muscle and the whole body composition had no significant differences between different vitamin E levels.The total antioxidant capacity (T-AOC) and superoxide dismutase (SOD) activity in liver increased at first and then decreased (P＜0.05) and both reached the maximum values at 31.86 mg/kg diet.Malondialdehyde (MDA) level in liver decreased significantly with the increase of dietary vitamin E level (P＜0.05).Liver vitamin E concentration increased linearly with the increase of dietary vitamin E (P＜0.05).【Conclusion】Using SGR as evaluation index,the dietary vitamin E requirement for juvenile Chinese sucker was 50.60 mg/kg based on broken-line regression analysis. |
| Key words: juvenile Chinese sucker vitamin E feed test |
