| 摘要: |
| 【目的】探索犬瘟热病毒 (Canine distemper virus，CDV)基因的遗传变异情况，为CDV的防控提供理论依据。【方法】收集2014-2015年流行于上海和安徽两地的CDV野毒株，用RT-PCR方法克隆其血球凝集素蛋白基因（H），从分子水平上讨论CDV H基因的流行规律、遗传进化特性和变异情况。【结果】分离的5株CDV之间H基因核苷酸和氨基酸序列的同源性分别为97.5%～99.9%和97.5%～99.2%，其与CDV疫苗株CDV3和Onderstepoort H基因核苷酸的同源性为90.9%～99.9%，氨基酸的同源性为90.4%～99.6%。进化树分析结果表明，分离到的5株CDV野毒株均属于亚洲Ⅰ型，与大部分的亚洲Ⅰ型处于同一分支。CDV H基因有9处潜在的天冬氨酸糖基化位点;H基因整体的同义突变概率与非同义突变概率的比例，即ds/dn=5.887 0。【结论】选择压力并未作用于分离到的CDV毒株，而是在中性选择作用下进化的。 |
| 关键词: 犬瘟热 H基因同源性 进化方式 天冬酰胺糖基化位点 |
| DOI： |
| 分类号: |
| 基金项目:国家自然科学基金项目(31270194)；上海市科委创新项目(13391901600) |
|
| Genetic analysis of H gene of canine distemper virus |
|
LI Dandan,QI Weiqiang,LI Chuanfeng,et al
|
| Abstract: |
| 【Objective】This article discussed the genetic characteristics and regularity of H gene from canine distemper virus (CDV) in molecular level.【Method】CDV strains from Shanghai and Anhui in 2014-2015 were collected and their H genes were cloned by RT-PCT.Then,the regularity and genetic characteristics were analyzed in molecular level.【Result】The nucleotide and amino acid similarities of H gene between the 5 wild strains were 97.5%-99.9% and 97.5%-99.2%,respectively.The nucleotide and amino acid similarities of H gene between the 5 wild strains and vaccine strains CDV3 and Onderstepoort were 90.9%-99.9% and 90.4%-99.6%.Phylogeny tree showed that the collected 5 CDV lineage belonged to Asia-Ⅰ,like most of the other H protein genes of Asia-Ⅰ.There are 9 potential N-glycosylation sites and ds/dn ratio was 5.887 0.【Conclusion】The selection process did not affect isolated CDV stains and the evolution occurred with a neutral evolution pattern. |
| Key words: canine distemper disease similarities of H gene evolution pattern N-glycosylation sites |
