| 摘要: |
| 【目的】研究G 蛋白偶联雌激素受体（G-protein-coupled estrogen receptor,GPER）对发情周期小鼠卵巢表皮生长因子受体（Epidermal growth factor receptor,EGFR）表达的影响。【方法】间情期小鼠腹腔注射GPER抑制剂G15，按注射剂量0.3，1.5和7.5 nmol/只设3个注射组，另设腹腔注射等体积生理盐水的对照组；药物注射后，依次取发情前期、发情期、发情后期和间情期小鼠的卵巢，用免疫组织化学SP 法和RT PCR法分别检测各组小鼠发情周期不同阶段卵巢EGFR及其mRNA的表达。【结果】EGFR免疫组化阳性产物主要分布于卵巢的各级卵泡中，阳性染色随卵泡发育而逐渐增强，其中颗粒细胞越靠近卵母细胞染色越强，发情周期卵巢EGFR相对表达量表现为发情期>发情前期>间情期>发情后期。小鼠注射G15后，各情期卵巢中EGFR的表达呈G15剂量依赖性减弱，除发情后期外，均极显著下降（P<0.01）；1.5和7.5 nmol/只 G15注射组各情期EGFR的相对表达量无显著差异（P>0.05），但均极显著低于0.3 nmol/只 G15注射组（P<0.01）。EGFR mRNA的变化规律与EGFR相对表达量的变化趋势完全一致。 【结论】G15可在一定程度上通过GPER下调发情周期小鼠卵巢中EGFR的表达，从而影响发情周期卵巢的功能。 |
| 关键词: 发情周期 卵巢 G蛋白偶联雌激素受体 G15 表皮生长因子受体 小鼠 |
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| 基金项目:国家林业局科研项目“野生动物疫源疫病监测及预警系统维护”（K313021501） |
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| Effects of G15 on expression of epidermal growth factor receptor in mice ovary during estrous cycle |
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YU Deshui,WANG Lu,LI Jing,et al
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| Abstract: |
| 【Objective】The purpose of this research was to investigate the effect of G-protein-coupled estrogen receptor (GPER,G15) on the expression of epidermal growth factor receptor (EGFR) in mice ovary during the estrous cycle.【Method】G15 was intraperitoneal injected to mice in 3 groups according to doses of 0.3,1.5 and 7.5 nmol,and mice in the control group were injected same volume of normal saline.After injection,the ovaries were removed during proestrus,estrus,dioestrus and metestrus stages.Immunohistochemistry and real-time quantitative RT-PCR were used to research the expression of EGFR and EGFR mRNA at different stages of estrous cycle.【Result】EGFR immunohistochemical positive products mainly distributed in ovarian follicles at different stages,positive staining strengthened gradually with follicular development,and those closer to the oocyte had deeper staining.The relative expression in ovarian estrous cycle was in the order of estrus>proestrus>dioestrus>metestrus.After G15 injection,the expressions of EGFR in each period showed G15 dose-dependent decrease very significantly (P<0.01) except for metestrus.The EGFR relative expressions of 1.5 and 7.5 nmol G15 injection groups in each period were significantly lower than that of 0.3 nmol G15 injection group (P<0.01),while no significant difference was found between these two groups (P>0.05).The trend of EGFR mRNA expression was consistent with the relative expression of and EGFR.【Conclusion】G15 inhibited the expression of EGFR by GPER in mice ovarian,and affected the function of ovarian during estrous cycle. |
| Key words: estrous cycle ovary G-protein-coupled estrogen receptor G15 epidermal growth factor receptor mice |
