| 摘要: |
| 【目的】探讨Ghrelin对绵羊早期胚胎发育的调节机制。【方法】将绵羊体外受精早期胚胎在Ghrelin组（300，1 000，10 000 ng/mL）、Ghrelin受体拮抗剂D-Lys3-GHRP-6组(10，200，400 ng/mL)、D-Lys3-GHRP-6+Ghrelin组（D-Lys3-GHRP-6 400 ng/mL，Ghrelin 300，1 000和2 000 ng/mL）、空白对照（不加D-Lys3-GHRP-6和Ghrelin）4个组别的胚胎发育液中进行发育培养，采用荧光定量PCR技术分别对各处理不同发育阶段（2-细胞期、4-细胞期、8-细胞期和16-细胞期）绵羊早期胚胎中ERK1、ERK2、P90rsk、Bcl-2、Bax mRNA的表达情况进行检测。【结果】与空白对照组相比，在添加Ghrelin组ERK1、ERK2、P90rsk、Bcl-2基因mRNA的表达表现为显著性上调（P＜0.05)，且这种上调作用可被D-Lys3-GHRP-6所拮抗；在添加Ghrelin组Bax基因mRNA的表达无显著性变化，但在D-Lys3-GHRP-6组Bax基因mRNA的表达表现为显著性上调（P＜0.05)。【结论】Ghrelin可级联激活MAPK信号通路，抑制促凋亡基因Bax的表达、促进抗凋亡基因Bcl-2的表达，进而促进绵羊早期胚胎的发育。 |
| 关键词: Ghrelin 绵羊 早期胚胎 MAPK 凋亡 |
| DOI： |
| 分类号: |
| 基金项目:国家自然科学基金项目（30860201） |
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| Regulation of early ovine embryos development in vitro by Ghrelin |
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YANG Yanyan,DA Lai,ZHANG Guanhua,et al
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| Abstract: |
| 【Objective】The purpose of this study was to investigate the regulation mechanism of ghrelin during the development of early ovine embryos.【Method】Early ovine embryos were cultured in four groups with different media:Ghrelin (300,1 000,and 10 000 ng/mL),D-Lys3-GHRP-6 (Ghrelin receptor antagonist;10,200,and 400 ng/mL),D-Lys3-GHRP-6 plus Ghrelin (D-Lys3-GHRP-6:400 ng/mL,Ghrelin 300,1 000,and 2 000 ng/mL),and the control group (D-Lys3-GHRP-6:0 ng/mL and Ghrelin 0 ng/mL).The mRNA expression levels of ERK1,ERK2,P90rsk,Bcl-2,and Bax were analyzed during the same stages（ 2-cell,4-cell,8-cell,and 16-cell）.【Result】Compared with the control group,mRNA expressions of ERK1,ERK2,P90rsk,and Bcl-2 were upregulated significantly (P<0.05),and the upregulated function could be blocked by D-Lys3-GHRP-6.Bax mRNA expression was insignificantly different in Ghrelin groups but it was upregulated significantly in D-Lys3-GHRP-6 group (P<0.05).【Conclusion】Ghrelin promoted the development of early ovine embryos by activing the MAPK pathway,inhibiting the expression of Bax gene and promoting the expression of Bcl-2 gene. |
| Key words: Ghrelin ovine embryo MAPK apoptosis |
