| 摘要: |
| 【目的】阐明华蟾毒精（CBG）对巨噬细胞功能的影响，为CBG的开发和利用提供理论依据。【方法】利用Balb/c小鼠制备与纯化腹腔巨噬细胞，采用不同质量浓度（0.5，1.0，1.5，2.0 mg/L）CBG作用于巨噬细胞，采用MTT法检测CBG对巨噬细胞吞噬金黄色葡萄球菌的影响；采用Griess法检测CBG对巨噬细胞分泌NO的影响；采用ELISA法检测CBG对巨噬细胞分泌IL-6、TNF-α和GM-CSF的影响。【结果】CBG质量浓度为1.0，1.5和2.0 mg/L组均能极显著提高巨噬细胞的吞噬功能；CBG质量浓度为0.5 mg/L 组能显著促进巨噬细胞NO的分泌，而 1.0，1.5和2.0 mg/L CBG组均能极显著促进巨噬细胞NO的分泌；CBG质量浓度为1.0，1.5和2.0 mg/L组均能极显著促进巨噬细胞IL-6的分泌；1.0 mg/L CBG组能显著促进巨噬细胞TNF-α的分泌，1.5和2.0 mg/L CBG组均能极显著促进巨噬细胞TNF-α的分泌；CBG质量浓度为0.5，1.0，1.5和2.0 mg/L组均能极显著促进巨噬细胞GM-CSF的分泌。【结论】CBG能提高巨噬细胞的吞噬功能和分泌功能，说明CBG具有良好的抗炎、抗肿瘤和提高免疫等作用。 |
| 关键词: 华蟾毒精 巨噬细胞 吞噬功能 分泌功能 |
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| 基金项目:辽宁省科技攻关计划项目(2014214016)；辽宁医学院校长基金 研究生科技创新基金项目(QM2014005) |
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| Effect of cinobufagin on macrophage function of mice |
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MENG Xianhua,YU Yang,HAO Lu,et al
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| Abstract: |
| 【Objective】This study investigated the effect of cinobugagin (CBG) on macrophage function of mice to provide basis for its development and utilization.【Method】Balb/c mice were used for preparation and purification of peritoneal macrophages,and CBG with different mass concentrations (0.5,1.0,1.5,2.0 mg/L) were applied on macrophages with blank control.After samples were taken,MTT method was used to detect effect on macrophage phagocytosis of staphylococcus aureus,Griess method was used to detect effect on NO secretion of macrophages,and ELISA method was used to test the effect on secretion of IL-6,TNF-α and GM-CSF.【Results】CBG groups with mass concentrations of 1.0,1.5,and 2.0 mg/L could significantly increase macrophage phagocytosis.CBG at concentration of 0.5 mg/L group significantly promoted NO secretion,and CBG at concentrations of 1.0,1.5,and 2.0 mg/L significantly promoted NO secretion.CBG at mass concentrations of 1.0,1.5,and 2.0 mg/L significantly promoted secretion of IL-6.CBG at mass concentration of 1.0 mg/L significantly promoted TNF-α secretion,and mass concentrations of 1.5 and 2.0 mg/L significantly promoted TNF-α secretion.CBG at mass concentrations of 0.5,1.0,1.5,and 2.0 mg/L significantly promoted GM-CSF secretion.【Conclusion】CBG can enhance macrophage phagocytosis function and secretion function,thus it has good function of anti-inflammatory,anti-tumor and improving immune. |
| Key words: cinobufagin macrophage phagocytosis function secretion funtion |
